HAF007). Specific bands were detected for PDK-1 at approximately 58-68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, Daudi human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and C6 rat glioma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PDK-1 Monoclonal Antibody (Catalog # MAB864) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). Specific bands were detected for PDK-1 at approximately 58-68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB864-SP | |||
HAF008). A specific band was detected for PDK-1 at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4." alt="Western blot shows lysates of WS-1 human fetal skin fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human PDK-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF864) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PDK-1 at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF864-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48631-25ul | |||
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for 
![Western Blot: PDK-1 [p Tyr9] Antibody [NBP3-23292] - Western blot image of A431 cells stimulated with pervanadate (lanes 1 and 3). The blots were then treated with akaline phosphatase (lanes 2 & 4) then probed with anti-PDK-1 (C-terminal region) mouse monoclonal antibody or anti-PDK-1 (Tyr-9) (NBP3-23292) rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/nbp3-23292_rabbit-pdk-1-p-tyr9-pab-19920231992816.jpg "<b>Biological Strategies Validation. </b>Western Blot: PDK-1 [p Tyr9] Antibody [NBP3-23292] - Western blot image of A431 cells stimulated with pervanadate (lanes 1 and 3). The blots were then treated with akaline phosphatase (lanes 2 & 4) then probed with anti-PDK-1 (C-terminal region) mouse monoclonal antibody or anti-PDK-1 (Tyr-9) (NBP3-23292) rabbit polyclonal antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PDK-1 [p Tyr9] Antibody [NBP3-23292] - Immunocytochemical labeling of PDK-1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK-1 (C-terminal Region) (NBP3-23291), rabbit polyclonal PDK-1 (Tyr-9) (NBP3-23292), and PDK-1 (N-terminus) (NBP3-23290) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23292_rabbit-pdk-1-p-tyr9-pab-19920231856946.jpg)
![Western Blot: PDK-1 Antibody [NBP3-23290] - Western blot image of A431 cells untreated (lanes 1 and 3) or treated with pervanadate (lanes 2, 4, 5 & 6). Blots were probed with anti-PDK-1 (NBP3-23290) or anti-PDK-1 (Tyr-9) (NBP3-23292). The latter was used in the presence of no peptide (lane 4), phospho-PDK-1 (Tyr-9) peptide (lane 5), or an unrelated phosphotyrosine peptide (lane 6).](http://images.novusbio.com/fullsize/nbp3-23290_rabbit-pdk-1-pab-19920231410127.jpg "<b>Biological Strategies Validation. </b>Western Blot: PDK-1 Antibody [NBP3-23290] - Western blot image of A431 cells untreated (lanes 1 and 3) or treated with pervanadate (lanes 2, 4, 5 & 6). Blots were probed with anti-PDK-1 (NBP3-23290) or anti-PDK-1 (Tyr-9) (NBP3-23292). The latter was used in the presence of no peptide (lane 4), phospho-PDK-1 (Tyr-9) peptide (lane 5), or an unrelated phosphotyrosine peptide (lane 6).")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PDK-1 Antibody [NBP3-23290] - Immunocytochemical labeling of PDK-1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK-1 (C-terminal Region) (NBP3-23291), rabbit polyclonal PDK-1 (Tyr-9) (NBP3-23292), and PDK-1 (N-terminus) (NBP3-23290) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23290_rabbit-pdk-1-pab-19920231252365.jpg)
![Immunocytochemistry/Immunofluorescence: PDK-1 Antibody (M146) [NBP3-23291] - Immunocytochemical labeling of PDK-1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK-1 (C-terminal Region) (NBP3-23291), rabbit polyclonal PDK-1 (Tyr-9) (NBP3-23292), and PDK-1 (N-terminus) (NBP3-23290) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23291_mouse-pdk-1-mab-m146-199202318202918.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PDK-1 Antibody (M146) [NBP3-23291] - Immunocytochemical labeling of PDK-1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK-1 (C-terminal Region) (NBP3-23291), rabbit polyclonal PDK-1 (Tyr-9) (NBP3-23292), and PDK-1 (N-terminus) (NBP3-23290) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.")
![<b>Biological Strategies Validation. </b>Western Blot: PDK-1 Antibody (M146) [NBP3-23291] - Western blot image of mouse brain (lane 1) and human A431 cells (lane 2). The blot was probed with anti-PDK-1 (C-terminal region) (NBP3-23291).](http://images.novusbio.com/fullsize/nbp3-23291_mouse-pdk-1-mab-m146-199202312523636.jpg)
![Western Blot: PDK-1 Antibody (2E2) [H00005170-M05] - Analysis of PDPK1 expression in rat brain.](http://images.novusbio.com/fullsize/PDK-1-Antibody-2E2-Western-Blot-H00005170-M05-img0002.jpg "Western Blot: PDK-1 Antibody (2E2) [H00005170-M05] - Analysis of PDPK1 expression in rat brain.")
![Western Blot: PDK-1 Antibody (2E2) [H00005170-M05] - Analysis of PDPK1 expression in IMR-32.](http://images.novusbio.com/fullsize/PDK-1-Antibody-2E2-Western-Blot-H00005170-M05-img0001.jpg)
![Western Blot: PDK-1 Antibody (2E2) [H00005170-M05] - Analysis of PDPK1 expression in transfected 293T cell line by PDPK1 monoclonal antibody (M05), clone 2E2. Lane 1: PDPK1 transfected lysate (Predicted MW: 48.2 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PDK-1-Antibody-2E2-Western-Blot-H00005170-M05-img0003.jpg)
![Western Blot: PDPK1 Antibody [NBP2-44132] - Detection of myc-tagged human PDK-1 at 60kDa in 10 Ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4 C with the](http://images.novusbio.com/fullsize/PDPK1-Antibody-Western-Blot-NBP2-44132-img0001.jpg "Western Blot: PDPK1 Antibody [NBP2-44132] - Detection of myc-tagged human PDK-1 at 60kDa in 10 Ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4 C with the")
![Western Blot: PDPK1 Overexpression Lysate (Adult Normal) [NBL1-14260] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PDPK1.](http://images.novusbio.com/fullsize/PDPK1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14260-img0002.jpg "Western Blot: PDPK1 Overexpression Lysate (Adult Normal) [NBL1-14260] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PDPK1.")
