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VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. Staining was performed using IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. Staining was performed using IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol." alt="NGFR/TNFRSF16 was detected in immersion fixed paraffin-embedded sections of human spinal cord tissue using Mouse Anti-Human NGFR/TNFRSF16 Monoclonal Antibody (Catalog # MAB3671) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. Staining was performed using IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol." class="big_thumb" />