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![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Spike Antibody [NBP3-07035] - Immunofluorescence Validation of SARS-CoV-2 Spike in HeLa cells. Immunofluorescence analysis of HeLa cells, untreated (right) and SARS-CoV-2 Spike plasmid transfected (left) using SARS-CoV-2 Spike antibody (NBP3-07035) at 1:100 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-07035-img0001.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Spike Antibody [NBP3-07035] - Immunofluorescence Validation of SARS-CoV-2 Spike in HeLa cells. Immunofluorescence analysis of HeLa cells, untreated (right) and SARS-CoV-2 Spike plasmid transfected (left) using SARS-CoV-2 Spike antibody (NBP3-07035) at 1:100 dilution. Blue: DAPI for nuclear staining.")
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-12852] - SARS-Cov-2 Spike 156-157EF Antibodies Specifically Do Not Recognize Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike 1566-157EF antibody, NBP3-12852, 1 ug/mL,, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-12852-img0001.jpg "ELISA: SARS-CoV-2 Spike Antibody [NBP3-12852] - SARS-Cov-2 Spike 156-157EF Antibodies Specifically Do Not Recognize Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike 1566-157EF antibody, NBP3-12852, 1 ug/mL,, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.")
![Western Blot: SARS-CoV-2 Spike Antibody [NBP3-12852] - WB Validation of of Spike 156-157EF Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Spike 156-157EF antibody, NBP3-12852, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr. SARS-CoV-2 Spike 156-157EF antibody (NBP3-12852) detects spike S1 protein of WT, but not Delta variant.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Western-Blot-NBP3-12852-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (CR3022) - Azide and BSA Free [NBP2-90980] - ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line) and SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using NBP2-90980; from 370 ng/ml for NBP3-11813 and from 10000 ng/ml for NBP3-11776. Human IgM and human IgA were HRP-conjugated and for the detection of human IgG1 a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.](http://images.novusbio.com/fullsize/nbp3-11776_human-monoclonal-sars-cov-2-spike-antibody-cr3022-7420252228564.png "ELISA: SARS-CoV-2 Spike Antibody (CR3022) - Azide and BSA Free [NBP2-90980] - ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line) and SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using NBP2-90980; from 370 ng/ml for NBP3-11813 and from 10000 ng/ml for NBP3-11776. Human IgM and human IgA were HRP-conjugated and for the detection of human IgG1 a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.")
![Western Blot: SARS-CoV-2 Spike RBD Antibody (B003Y) - (RBD) [NBP3-05726] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B003Y-RBD-Western-Blot-NBP3-05726-img0001.jpg "Western Blot: SARS-CoV-2 Spike RBD Antibody (B003Y) - (RBD) [NBP3-05726] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (B003Y) - (RBD) [NBP3-05726] - ELISA Test. Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 (COVID-19) Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike RBD Antibody, NBP3-05726. Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution. Develop: 15min, 100 uL/well. Stop: Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B003Y-RBD-ELISA-NBP3-05726-img0002.jpg)
![Western Blot: SARS-CoV-2 Spike RBD Antibody (B003N) - (RBD) [NBP3-05727] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B003N-RBD-Western-Blot-NBP3-05727-img0001.jpg "Western Blot: SARS-CoV-2 Spike RBD Antibody (B003N) - (RBD) [NBP3-05727] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (B003N) - (RBD) [NBP3-05727] - ELISA Test. Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 (COVID-19) Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike RBD Antibody, NBP3-05727. Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution. Develop: 15min, 100 uL/well. Stop: Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B003N-RBD-ELISA-NBP3-05727-img0002.jpg)
![Western Blot: SARS-CoV-2 Spike Antibody (B004Y) - (ECD) [NBP3-05723] - Western Blot Validation with Recombinant Protein. Loading: 1ug of recombinant protein per lane. Lane 1: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike, NBP3-05723, 1:500. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-B004Y-ECD-Western-Blot-NBP3-05723-img0002.jpg "Western Blot: SARS-CoV-2 Spike Antibody (B004Y) - (ECD) [NBP3-05723] - Western Blot Validation with Recombinant Protein. Loading: 1ug of recombinant protein per lane. Lane 1: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike, NBP3-05723, 1:500. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.")
![ELISA: SARS-CoV-2 Spike Antibody (B004Y) - (ECD) [NBP3-05723] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-RBD Recombinant Protein, and column 2: SARS-CoV-2 Spike-ECD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.10-563 doesn't react with 10-008.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-B004Y-ECD-ELISA-NBP3-05723-img0001.jpg)
![Western Blot: SARS-CoV-2 Spike Antibody (B004N) - (ECD) [NBP3-05724] - Western Blot Validation with Recombinant Protein. Loading: 1ug of recombinant protein per lane. Lane 1: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike, NBP3-05724, 1:500. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-B004N-ECD-Western-Blot-NBP3-05724-img0002.jpg "Western Blot: SARS-CoV-2 Spike Antibody (B004N) - (ECD) [NBP3-05724] - Western Blot Validation with Recombinant Protein. Loading: 1ug of recombinant protein per lane. Lane 1: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike, NBP3-05724, 1:500. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.")
![ELISA: SARS-CoV-2 Spike Antibody (B004N) - (ECD) [NBP3-05724] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-RBD Recombinant Protein, and column 2: SARS-CoV-2 Spike-ECD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.+.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-B004N-ECD-ELISA-NBP3-05724-img0001.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (T4P5-H12) - VHH [NBP3-12848] - A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, S1, ECD) as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12848) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12848 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-T4P5-H12-VHH-ELISA-NBP3-12848-img0001.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (T4P5-H12) - VHH [NBP3-12848] - A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, S1, ECD) as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12848) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12848 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (T4P5-H12) - VHH - Azide and BSA Free [NBP3-12848] - Validation with SARS-CoV-2 (COVID-19) Spike Trimer Proteins. Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, . A direct ELISA was performed using SARS-CoV-2 Spike trimer recombinant proteins of WT, Omicron and Delta variants as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibody as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution. Detection range is from 0.4 ng/mL to 900 ng/mL. SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, can detect spike trimer protein at 1.5 ng/mL.](http://images.novusbio.com/fullsize/nbp3-12848_llama-monoclonal-sars-cov-2-spike-rbd-antibody-t4p5-h12-vhh-2711202420392741.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (T4P5-H12) - VHH - Azide and BSA Free [NBP3-12848] - Validation with SARS-CoV-2 (COVID-19) Spike Trimer Proteins of Omicron and Delta Variants. Antibodies: SARS-CoV-2 Spike RBD Antibodies. A direct ELISA was performed using SARS-CoV-2 Spike Trimer recombinant proteins of Omicron and Delta variants as coating antigen at 1 ug/mL and each of the 6 anti-SARS-CoV-2 Spike RBD antibodies as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SD9439, SD9441, and SD9503 bind spike proteins of omicron variant, but not delta variant; SD9511 and SD9437 bind spike proteins of delta variant, but not omicron variant; binds spike proteins of delta and omicron variants.](http://images.novusbio.com/fullsize/nbp3-12848_llama-monoclonal-sars-cov-2-spike-rbd-antibody-t4p5-h12-vhh-2711202419432256.jpg)
![Western Blot: SARS-CoV-2 Spike Antibody (B005N) - (ECD) [NBP3-05722] - Western Blot Validation with Recombinant Protein. Loading: 1ug of recombinant protein per lane. Lane 1: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike, NBP3-05722, 1:4000. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-B005N-ECD-Western-Blot-NBP3-05722-img0002.jpg "Western Blot: SARS-CoV-2 Spike Antibody (B005N) - (ECD) [NBP3-05722] - Western Blot Validation with Recombinant Protein. Loading: 1ug of recombinant protein per lane. Lane 1: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike, NBP3-05722, 1:4000. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.")
![ELISA: SARS-CoV-2 Spike Antibody (B005N) - (ECD) [NBP3-05722] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-RBD Recombinant Protein, and column 2: SARS-CoV-2 Spike-ECD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-B005N-ECD-ELISA-NBP3-05722-img0001.jpg)
![Western Blot: SARS-CoV-2 Spike RBD Antibody (B001Y) - (RBD) [NBP3-05725] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B001Y-RBD-Western-Blot-NBP3-05725-img0001.jpg "Western Blot: SARS-CoV-2 Spike RBD Antibody (B001Y) - (RBD) [NBP3-05725] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (B001Y) - (RBD) [NBP3-05725] - ELISA Test. Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 (COVID-19) Spike-ECD Recombinant Protein and column 2: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike RBD Antibody, NBP3-05725. Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution. Develop: 15min, 100 uL/well. Stop: Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B001Y-RBD-ELISA-NBP3-05725-img0002.jpg)
![Western Blot: SARS-CoV-2 Spike RBD Antibody (B001N) - (RBD) [NBP3-05728] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B001N-RBD-Western-Blot-NBP3-05728-img0001.jpg "Western Blot: SARS-CoV-2 Spike RBD Antibody (B001N) - (RBD) [NBP3-05728] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (B001N) - (RBD) [NBP3-05728] - ELISA Test. Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 (COVID-19) Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike RBD Antibody, NBP3-05728. Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution. Develop: 15min, 100 uL/well. Stop: Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B001N-RBD-ELISA-NBP3-05728-img0002.jpg)
![Western Blot: SARS-CoV-2 Spike S2 Antibody [NBP3-05700] - Validation with SARS-CoV-2 Spike S2 ECD Recombinant Protein Loading: 30 ng per lane of SARS-CoV-2 Spike S2 ECD recombinant protein, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-S2-Antibody-Western-Blot-NBP3-05700-img0001.jpg "Western Blot: SARS-CoV-2 Spike S2 Antibody [NBP3-05700] - Validation with SARS-CoV-2 Spike S2 ECD Recombinant Protein Loading: 30 ng per lane of SARS-CoV-2 Spike S2 ECD recombinant protein, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL.")
![ELISA: SARS-CoV-2 Spike S2 Antibody - BSA Free [NBP3-05700] - Figure 2 ELISA Validation Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, 9181. A direct ELISA was performed using immunogen peptide (P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.](http://images.novusbio.com/fullsize/nbp3-05700_rabbit-polyclonal-sars-cov-2-spike-s2-antibody-2711202420414569.jpg)