| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26841-25ul | |||
| Azide and BSA Free | NBP3-44286 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10395-SP | |||
| Carrier Free | FAB10395V-100UG | |||
| Carrier Free | FAB10395U-100UG | |||
| Carrier Free | FAB10395T-100UG | |||
| Carrier Free | FAB10395S-100UG | |||
| Carrier Free | FAB10395R-100UG | |||
| Carrier Free | FAB10395N-100UG | |||
| Carrier Free | FAB10395G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26839-25ul | |||
| Azide and BSA Free | NBP3-44285 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2319-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | MAB23191-SP | |||
![Western Blot: LAG-3 Antibody (PSH0-44) [NBP3-32515] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32515) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 55/70 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32515) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32515_rabbit-lag-3-mab-psh0-44-278202415212421.jpg "Western Blot: LAG-3 Antibody (PSH0-44) [NBP3-32515] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32515) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 55/70 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32515) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAG-3 Antibody (PSH0-44) [NBP3-32515] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-LAG-3 antibody (NBP3-32515) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32515) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32515_rabbit-lag-3-mab-psh0-44-29820241435254.jpg)
![Immunocytochemistry/ Immunofluorescence: LAG-3 Antibody (PSH0-44) [NBP3-32515] - Immunocytochemistry analysis of NIH/3T3 cells transfected with or without LAG3 labeling LAG-3 with Rabbit anti-LAG-3 antibody (NBP3-32515) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LAG-3 antibody (NBP3-32515) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32515_rabbit-lag-3-mab-psh0-44-2920241033400.jpg)
![Western Blot: LAG-3 Antibody (PS01-33) [NBP3-32516] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/1,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 57/70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32516) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32516_rabbit-lag-3-mab-ps01-33-2882024142261.jpg "Western Blot: LAG-3 Antibody (PS01-33) [NBP3-32516] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/1,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 57/70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32516) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAG-3 Antibody (PS01-33) [NBP3-32516] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32516) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32516_rabbit-lag-3-mab-ps01-33-29820241416185.jpg)
![Immunocytochemistry/ Immunofluorescence: LAG-3 Antibody (PS01-33) [NBP3-32516] - Immunocytochemistry analysis of NIH/3T3 cells transfected with or without LAG-3 labeling LAG-3 with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32516_rabbit-lag-3-mab-ps01-33-29202410334064.jpg)
