Products Related to Gene-ID: 3592

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL‑12 p70 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line treated with 5 ng/mL PMA for 48 hours and 100 ng/mL LPS and 3 µM monensin for 24 hours (positive staining) and THP‑1 human acute monocytic leukemia cell line (untreated, negative staining) using Mouse Anti-Human IL‑12 p70 Monoclonal Antibody (Catalog # MAB611R) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

219-IL) was serially diluted and captured by Mouse Anti-Human IL‑12 p70 Monoclonal Antibody (Catalog # MAB611R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 219-IL) was serially diluted and captured by Mouse Anti-Human IL‑12 p70 Monoclonal Antibody (Catalog # MAB611R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />