HAF005). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast cancer cell line untreated (-) or treated (+) with 20 mJ/cm2ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.25 µg/mL of Rat Anti-Human HSP27 (S78/S82) Monoclonal Antibody (Catalog # MAB23142) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB23142-SP | |||
![Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (NBP3-13297) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/HSP27-[p-Ser82]-Antibody-Western-Blot-NBP3-13297-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (NBP3-13297) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (NBP3-13297) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP27-[p-Ser82]-Antibody-Western-Blot-NBP3-13297-img0001.jpg)
![Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (NBP3-13297) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/HSP27-[p-Ser82]-Antibody-Western-Blot-NBP3-13297-img0002.jpg)
![Western Blot: HSP27 Antibody (S03-9F4) [NBP3-20041] - Western blot detection of HSP27 in Hela cell lysates using NBP3-20041 (1:1000 diluted). Predicted band size: 23kDa. Observed band size: 27kDa.](http://images.novusbio.com/fullsize/HSP27-Antibody-S03-9F4-Western-Blot-NBP3-20041-img0002.jpg "Western Blot: HSP27 Antibody (S03-9F4) [NBP3-20041] - Western blot detection of HSP27 in Hela cell lysates using NBP3-20041 (1:1000 diluted). Predicted band size: 23kDa. Observed band size: 27kDa.")
![Immunohistochemistry-Paraffin: HSP27 Antibody (S03-9F4) [NBP3-20041] - Immunohistochemical of HSP27 in Human breast cancer tissue using HNBP3-20041 at dilution 1/50](http://images.novusbio.com/fullsize/HSP27-Antibody-S03-9F4-Immunohistochemistry-Paraffin-NBP3-20041-img0001.jpg)
![ELISA: Rat HSP27 ELISA Kit (Colorimetric) [NBP2-76439] - Samples were spiked with high concentrations of Rat HSP27 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76439_rat-hsp27-elisa-kit-colorimetric-1322024162040.jpg "ELISA: Rat HSP27 ELISA Kit (Colorimetric) [NBP2-76439] - Samples were spiked with high concentrations of Rat HSP27 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat HSP27 ELISA Kit (Colorimetric) [NBP2-76439] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76439_rat-hsp27-elisa-kit-colorimetric-432024983599.jpg)
