| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70937 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3317-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3317-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-68846-25ul | |||
![Western Blot: HES-1 Antibody (OTI4H1) [NBP1-47791] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1 (1:500).](http://images.novusbio.com/fullsize/HES-1-Antibody-OTI4H1-Western-Blot-NBP1-47791-img0018.jpg "Western Blot: HES-1 Antibody (OTI4H1) [NBP1-47791] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1 (1:500).")
![Immunocytochemistry/Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791] - HeLa cells using anti-HES1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HES-1-Antibody-OTI4H1-Immunocytochemistry-Immunofluorescence-NBP1-47791-img0010.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded liver using anti-Hes1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HES-1-Antibody-OTI4H1-Immunohistochemistry-Paraffin-NBP1-47791-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hes1.](http://images.novusbio.com/fullsize/HES-1-Antibody-OTI4H1-Immunocytochemistry-Immunofluorescence-NBP1-47791-img0008.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded breast using anti-Hes1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HES-1-Antibody-OTI4H1-Immunohistochemistry-Paraffin-NBP1-47791-img0011.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded Carcinoma of liver using anti-Hes1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HES-1-Antibody-OTI4H1-Immunohistochemistry-Paraffin-NBP1-47791-img0012.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded endometrium using anti-Hes1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HES-1-Antibody-OTI4H1-Immunohistochemistry-Paraffin-NBP1-47791-img0013.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded kidney using anti-Hes1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HES-1-Antibody-OTI4H1-Immunohistochemistry-Paraffin-NBP1-47791-img0014.jpg)
![Western Blot: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of Hes1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysate Lane 2: SK-Br-3 cell lysate](http://images.novusbio.com/fullsize/HES-1-Antibody-SC06-21-Western-Blot-NBP2-67642-img0005.jpg "Western Blot: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of Hes1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysate Lane 2: SK-Br-3 cell lysate")
![Immunocytochemistry/Immunofluorescence: HES-1 Antibody (SC06-21) [NBP2-67642] - Staining Hes1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/HES-1-Antibody-SC06-21-Immunocytochemistry-Immunofluorescence-NBP2-67642-img0003.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-HES-1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/HES-1-Antibody-SC06-21-Immunohistochemistry-Paraffin-NBP2-67642-img0007.jpg)
![Flow Cytometry: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of sh-sy-5y cells with Hes1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/HES-1-Antibody-SC06-21-Flow-Cytometry-NBP2-67642-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HES-1 Antibody (SC06-21) [NBP2-67642] - Staining Hes1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/HES-1-Antibody-SC06-21-Immunocytochemistry-Immunofluorescence-NBP2-67642-img0002.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of paraffin-embedded mouse brain tissue using anti-Hes1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HES-1-Antibody-SC06-21-Immunohistochemistry-Paraffin-NBP2-67642-img0004.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of paraffin-embedded rat brain tissue using anti-Hes1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/HES-1-Antibody-SC06-21-Immunohistochemistry-Paraffin-NBP2-67642-img0006.jpg)
![Western Blot: HES-1 Antibody (9I2E8) [NBP3-15320] - Western blot analysis of extracts of various cell lines, using HES-1 Rabbit mAb (NBP3-15320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/HES-1-Antibody-9I2E8-Western-Blot-NBP3-15320-img0003.jpg "Western Blot: HES-1 Antibody (9I2E8) [NBP3-15320] - Western blot analysis of extracts of various cell lines, using HES-1 Rabbit mAb (NBP3-15320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: HES-1 Antibody (9I2E8) [NBP3-15320] - Immunohistochemistry of paraffin-embedded mouse spinal cord using HES-1 Rabbit mAb (NBP3-15320) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HES-1-Antibody-9I2E8-Immunohistochemistry-Paraffin-NBP3-15320-img0002.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (9I2E8) [NBP3-15320] - Immunohistochemistry of paraffin-embedded rat brain using HES-1 Rabbit mAb (NBP3-15320) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HES-1-Antibody-9I2E8-Immunohistochemistry-Paraffin-NBP3-15320-img0001.jpg)
![Immunoprecipitation: HES-1 Antibody (9I2E8) [NBP3-15320] - Immunoprecipitation analysis of 600ug extracts of Rat testis cells using 3ug HES-1 antibody (NBP3-15320). Western blot was performed from the immunoprecipitate using HES-1 antibody (NBP3-15320) at a dilition of 1:500.](http://images.novusbio.com/fullsize/HES-1-Antibody-9I2E8-Immunoprecipitation-NBP3-15320-img0004.jpg)
![Immunohistochemistry: HES-1 Antibody (9I2E8) [HES-1] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using HES-1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15320_rabbit-hes-1-mab-9i2e8-52202517202934.jpg)
![Immunohistochemistry: HES-1 Antibody (9I2E8) [HES-1] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using HES-1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15320_rabbit-hes-1-mab-9i2e8-52202517284991.jpg)
![Immunohistochemistry: HES-1 Antibody (9I2E8) [HES-1] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using HES-1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15320_rabbit-hes-1-mab-9i2e8-52202517192932.jpg)
![Immunohistochemistry: HES-1 Antibody (9I2E8) [HES-1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using HES-1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15320_rabbit-hes-1-mab-9i2e8-52202517192922.jpg)
![Immunohistochemistry: HES-1 Antibody (9I2E8) [HES-1] - Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using HES-1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15320_rabbit-hes-1-mab-9i2e8-52202517184531.jpg)
![Western Blot: HES-1 Antibody [NBP1-30912] - PC-12 whole cell extract.12 % SDS-PAGE HES1 antibody [N1], N-term dilution: 1:1000](http://images.novusbio.com/fullsize/HES-1-Antibody-Western-Blot-NBP1-30912-img0012.jpg "Western Blot: HES-1 Antibody [NBP1-30912] - PC-12 whole cell extract.12 % SDS-PAGE HES1 antibody [N1], N-term dilution: 1:1000")
![Immunoprecipitation: HES-1 Antibody [NBP1-30912] - A431 whole cell lysate/extract A : Control with 2.5 ug of pre-immune rabbit IgG B : Immunoprecipitation of HES1 by 2.5 ug of HES1 antibody 12% SDS-PAGE The immunoprecipitated HES1 protein was detected by HES1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HES-1-Antibody-Immunoprecipitation-NBP1-30912-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: HES-1 Antibody [NBP1-30912] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-30912_rabbit-polyclonal-hes-1-antibody-1610202419405759.jpg)
![HES-1 antibody [N1], N-term detects HES-1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HES-1 protein stained by HES-1 antibody [N1], N-term (NBP1-30912) diluted at 1:2000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-30912_rabbit-polyclonal-hes-1-antibody-immunocytochemistry-immunofluorescence-141120251837384.jpg)
![MCF-7 whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HES-1 antibody [N1], N-term (NBP1-30912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30912_rabbit-polyclonal-hes-1-antibody-western-blot-141120251843132.jpg)
![Immunocytochemistry/Immunofluorescence: HES-1 Antibody (HL2198) - Azide and BSA Free [NBP3-25501] - HES1 antibody [HL2198] detects HES1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HES1 stained by HES1 antibody [HL2198] (NBP3-25501) diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25501_rabbit-hes-1-mab-hl2198-azide-and-bsa-free-2120249371579.jpg "Immunocytochemistry/Immunofluorescence: HES-1 Antibody (HL2198) - Azide and BSA Free [NBP3-25501] - HES1 antibody [HL2198] detects HES1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HES1 stained by HES1 antibody [HL2198] (NBP3-25501) diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: HES-1 Antibody (HL2198) - Azide and BSA Free [NBP3-25501] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [HL2198] (NBP3-25501) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25501_rabbit-hes-1-mab-hl2198-azide-and-bsa-free-2120249403841.jpg)
![Western Blot: HES-1 Antibody (HL2198) - Azide and BSA Free [NBP3-25501] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [HL2198] (NBP3-25501) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25501_rabbit-hes-1-mab-hl2198-azide-and-bsa-free-2120249425460.jpg)
![Western Blot: HES-1 Antibody (HL2198) - Azide and BSA Free [NBP3-25501] - MCF-7 whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [HL2198] (NBP3-25501) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25501_rabbit-hes-1-mab-hl2198-azide-and-bsa-free-2120249355570.jpg)
![Western Blot: HES-1 Antibody (3A3) [H00003280-M02] - HES1 monoclonal antibody (M02), clone 3A3. Analysis of HES1 expression in H9c2(2-1).](http://images.novusbio.com/fullsize/HES-1-Antibody-3A3-Western-Blot-H00003280-M02-img0007.jpg "Western Blot: HES-1 Antibody (3A3) [H00003280-M02] - HES1 monoclonal antibody (M02), clone 3A3. Analysis of HES1 expression in H9c2(2-1).")
![Immunocytochemistry/Immunofluorescence: HES-1 Antibody (3A3) [H00003280-M02] - Analysis of monoclonal antibody to HES1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/HES-1-Antibody-3A3-Immunocytochemistry-Immunofluorescence-H00003280-M02-img0006.jpg)
![Western Blot: HES-1 Antibody [NBP2-68846] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/HES-1-Antibody-Western-Blot-NBP2-68846-img0001.jpg "Western Blot: HES-1 Antibody [NBP2-68846] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: HES-1 Antibody [NBP2-68846] - Staining of human cell line U-2 OS shows localization to nucleus.](http://images.novusbio.com/fullsize/HES-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68846-img0002.jpg)
![Western Blot: Hes1 Overexpression Lysate (Adult Normal) [NBP2-07738] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hes1.](http://images.novusbio.com/fullsize/Hes1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07738-img0001.jpg "Western Blot: Hes1 Overexpression Lysate (Adult Normal) [NBP2-07738] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hes1.")