| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47464-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04637-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04638-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57061-25ul | |||
![Western Blot: EYA3 Antibody [NBP1-46818] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EYA3 antibody NBP1-46818 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. EYA3 was also immunoprecipitated by rabbit anti-EYA3 antibody NBP1-46819, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/EYA3-Antibody-Western-Blot-NBP1-46818-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: EYA3 Antibody [NBP1-46818] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EYA3 antibody NBP1-46818 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. EYA3 was also immunoprecipitated by rabbit anti-EYA3 antibody NBP1-46819, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: EYA3 Antibody [NBP1-46818] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EYA3 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EYA3-Antibody-Immunohistochemistry-NBP1-46818-img0006.jpg)
![Immunohistochemistry-Paraffin: EYA3 Antibody [NBP1-46818] - FFPE section of human colon carcinoma. Antibody used at a dilution of 1:250 Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/EYA3-Antibody-Immunohistochemistry-Paraffin-NBP1-46818-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: EYA3 Antibody [NBP2-47464] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/EYA3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47464-img0003.jpg "Immunocytochemistry/Immunofluorescence: EYA3 Antibody [NBP2-47464] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: EYA3 Antibody [NBP2-47464] - Staining of human colon tissue. Shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/EYA3-Antibody-Immunohistochemistry-NBP2-47464-img0001.jpg)
![Western Blot: EYA3 Antibody [NBP3-04637] - Western blot analysis of extracts of various cell lines, using EYA3 antibody (NBP3-04637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/EYA3 Antibody-Western Blot-NBP3-04637-img0002.jpg "Western Blot: EYA3 Antibody [NBP3-04637] - Western blot analysis of extracts of various cell lines, using EYA3 antibody (NBP3-04637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: EYA3 Antibody - BSA Free [NBP3-04637] - Immunofluorescence analysis of U2OS cells using EYA3 antibody (A15056) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04637_rabbit-polyclonal-eya3-antibody-5220251931320.jpg)
![Western Blot: EYA3 Antibody [NBP3-04638] - Analysis of extracts of various cell lines, using EYA3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/EYA3-Antibody-Western-Blot-NBP3-04638-img0005.jpg "Western Blot: EYA3 Antibody [NBP3-04638] - Analysis of extracts of various cell lines, using EYA3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunocytochemistry/Immunofluorescence: EYA3 Antibody [NBP2-57061] - Staining of human cell line RH-30 shows localization to nucleoplasm & centrosome.](http://images.novusbio.com/fullsize/EYA3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57061-img0001.jpg "Immunocytochemistry/Immunofluorescence: EYA3 Antibody [NBP2-57061] - Staining of human cell line RH-30 shows localization to nucleoplasm & centrosome.")
![Immunoprecipitation: EYA3 Antibody [NBP1-46819] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EYA3 antibody NBP1-46819 used for IP at 3 ug/mg lysate. EYA3 was also immunoprecipitated by rabbit anti-EYA3 antibody NBP1-46818, which recognizes an upstream epitope. For blotting immunoprecipitated EYA3, NBP1-46818 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/EYA3-Antibody-Immunoprecipitation-NBP1-46819-img0001.jpg "Immunoprecipitation: EYA3 Antibody [NBP1-46819] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EYA3 antibody NBP1-46819 used for IP at 3 ug/mg lysate. EYA3 was also immunoprecipitated by rabbit anti-EYA3 antibody NBP1-46818, which recognizes an upstream epitope. For blotting immunoprecipitated EYA3, NBP1-46818 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")