HAF018). A specific band was detected for Fetuin A/AHSG at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of rat liver tissue and rat lung tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Rat Fetuin A/AHSG Monoclonal Antibody (Catalog # MAB7510) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fetuin A/AHSG at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7510-SP | |||
HAF016). A specific band was detected for Fetuin A/AHSG at approximately 59-64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of rat liver tissue and rat lung tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Rat Fetuin A/AHSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7510) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fetuin A/AHSG at approximately 59-64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7510-SP | |||
1184-PI) was serially diluted and captured by Mouse Anti-Human Fetuin A/AHSG Monoclonal Antibody (Catalog # MAB11841) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Fetuin A/AHSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1184) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human Fetuin A/AHSG (Catalog # 1184-PI) was serially diluted and captured by Mouse Anti-Human Fetuin A/AHSG Monoclonal Antibody (Catalog # MAB11841) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Fetuin A/AHSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1184) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB11841-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1563-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-79670-5Plates | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22369-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-79505-5Plates | |||
![Sandwich ELISA: Fetuin A/AHSG Antibody Pair [HRP] [NBP2-79670] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/Fetuin-A-AHSG-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79670-img0001.jpg "Sandwich ELISA: Fetuin A/AHSG Antibody Pair [HRP] [NBP2-79670] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
![Immunohistochemistry: Fetuin A/AHSG Antibody (JE54-96) [NBP3-32336] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Fetuin A/AHSG antibody (NBP3-32336) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32336) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32336_rabbit-fetuin-a-ahsg-mab-je54-96-298202414161872.jpg "Immunohistochemistry: Fetuin A/AHSG Antibody (JE54-96) [NBP3-32336] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Fetuin A/AHSG antibody (NBP3-32336) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32336) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: Fetuin A/AHSG Antibody (JE54-96) [NBP3-32336] - Flow cytometric analysis of HepG2 cells labeling Fetuin A/AHSG.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32336, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32336_rabbit-fetuin-a-ahsg-mab-je54-96-29202416453229.jpg)
![ELISA: Rat Fetuin A/AHSG ELISA Kit (Colorimetric) [NBP2-78752] - Samples were spiked with high concentrations of Rat Fetuin A/AHSG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-78752_rat-fetuin-a-ahsg-elisa-kit-colorimetric-13220241613098.jpg "ELISA: Rat Fetuin A/AHSG ELISA Kit (Colorimetric) [NBP2-78752] - Samples were spiked with high concentrations of Rat Fetuin A/AHSG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Fetuin A/AHSG ELISA Kit (Colorimetric) [NBP2-78752] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-78752_rat-fetuin-a-ahsg-elisa-kit-colorimetric-43202495506.jpg)
![Western Blot: Fetuin Antibody [NBP1-02152] - Analysis of fetuin in purified preparations and in human plasma. Lane 1 contains 250 ng of purified human fetuin. Lane 2 contains 5 l of a 1:50 dilution of human serum. Dilution of Anti-Human Fetuin antibody between 1:10,000 and 1:20,000 showed strong reactivity by western blot. In this blot the antibody was used at a 1:10,000 dilution incubated 1 h at room temperature in 1% BSA in TTBS. Detection occurred using a 1:5,000 dilution of IRDye800 conjugated Donkey anti-Goat IgG for 45 min at room temperature.](http://images.novusbio.com/fullsize/Fetuin-Antibody-Western-Blot-NBP1-02152-img0003.jpg "Western Blot: Fetuin Antibody [NBP1-02152] - Analysis of fetuin in purified preparations and in human plasma. Lane 1 contains 250 ng of purified human fetuin. Lane 2 contains 5 l of a 1:50 dilution of human serum. Dilution of Anti-Human Fetuin antibody between 1:10,000 and 1:20,000 showed strong reactivity by western blot. In this blot the antibody was used at a 1:10,000 dilution incubated 1 h at room temperature in 1% BSA in TTBS. Detection occurred using a 1:5,000 dilution of IRDye800 conjugated Donkey anti-Goat IgG for 45 min at room temperature.")
![Western Blot: Alpha 2 HS Glycoprotein Overexpression Lysate (Adult Normal) [NBL1-07405] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Alpha 2 HS Glycoprotein.](http://images.novusbio.com/fullsize/Alpha-2-HS-Glycoprotein-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07405-img0002.jpg "Western Blot: Alpha 2 HS Glycoprotein Overexpression Lysate (Adult Normal) [NBL1-07405] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Alpha 2 HS Glycoprotein.")
![Bovine Fetuin A/AHSG ELISA Kit (Colorimetric) [NBP2-77017] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-77017_bovine-fetuin-a-ahsg-elisa-kit-colorimetric-3120248343417.png "Bovine Fetuin A/AHSG ELISA Kit (Colorimetric) [NBP2-77017] - Standard Curve Reference")
![Western Blot: Fetuin A/AHSG Antibody (SR2026) [NBP3-22369] - Western blot analysis using NBP3-22369 in Human plasma lysate.](http://images.novusbio.com/fullsize/nbp3-22369_rabbit-fetuin-a-ahsg-mab-sr2026-57202316582148.jpg "Western Blot: Fetuin A/AHSG Antibody (SR2026) [NBP3-22369] - Western blot analysis using NBP3-22369 in Human plasma lysate.")
![Sandwich ELISA: Fetuin A/AHSG Antibody Pair [HRP] [NBP2-79505] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/Fetuin-A-AHSG-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79505-img0001.jpg "Sandwich ELISA: Fetuin A/AHSG Antibody Pair [HRP] [NBP2-79505] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
