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![Immunohistochemistry-Paraffin: HB-EGF Antibody [NBP2-14082] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/HB-EGF-Antibody-Immunohistochemistry-Paraffin-NBP2-14082-img0005.jpg "Immunohistochemistry-Paraffin: HB-EGF Antibody [NBP2-14082] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.")
![Immunohistochemistry-Paraffin: HB-EGF Antibody [NBP2-14082] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/HB-EGF-Antibody-Immunohistochemistry-Paraffin-NBP2-14082-img0002.jpg)
![Immunohistochemistry-Paraffin: HB-EGF Antibody [NBP2-14082] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/HB-EGF-Antibody-Immunohistochemistry-Paraffin-NBP2-14082-img0003.jpg)
![Immunohistochemistry-Paraffin: HB-EGF Antibody [NBP2-14082] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/HB-EGF-Antibody-Immunohistochemistry-Paraffin-NBP2-14082-img0004.jpg)
![Western Blot: HB-EGF Antibody (JE34-79) [NBP3-32418] - Western blot analysis of HB-EGF on different lysates with Rabbit anti-HB-EGF antibody (NBP3-32418) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: 293T cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32418) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32418_rabbit-hb-egf-mab-je34-79-278202416331232.jpg "Western Blot: HB-EGF Antibody (JE34-79) [NBP3-32418] - Western blot analysis of HB-EGF on different lysates with Rabbit anti-HB-EGF antibody (NBP3-32418) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: 293T cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32418) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HB-EGF Antibody (JE34-79) [NBP3-32418] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-HB-EGF antibody (NBP3-32418) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32418) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32418_rabbit-hb-egf-mab-je34-79-29820241435221.jpg)
![ELISA: Mouse HB-EGF ELISA Kit (Chemiluminescence) [NBP2-62780] - Samples were spiked with high concentrations of Mouse HB-EGF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62780_mouse-hb-egf-elisa-kit-chemiluminescence-132202416221037.jpg)
![ELISA: Mouse HB-EGF ELISA Kit (Chemiluminescence) [NBP2-62780] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62780_mouse-hb-egf-elisa-kit-chemiluminescence-432024983560.jpg)
![Western Blot: HB-EGF Antibody (9C2M6) [NBP3-15422] - Western blot analysis of extracts of various cell lines, using HB-EGF Rabbit mAb (NBP3-15422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/HB-EGF-Antibody-9C2M6-Western-Blot-NBP3-15422-img0002.jpg "Western Blot: HB-EGF Antibody (9C2M6) [NBP3-15422] - Western blot analysis of extracts of various cell lines, using HB-EGF Rabbit mAb (NBP3-15422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: HB-EGF Antibody (S08-9C2) [NBP3-15039] - Western blot detection of HB-EGF in K562, C6, 3T3, Hela cell lysates using HB-EGF Rabbit mAb (1:1000 diluted). Predicted band size: 23kDa. Observed band size: 23kDa.](http://images.novusbio.com/fullsize/HB-EGF-Antibody-S08-9C2-Western-Blot-NBP3-15039-img0001.jpg "Western Blot: HB-EGF Antibody (S08-9C2) [NBP3-15039] - Western blot detection of HB-EGF in K562, C6, 3T3, Hela cell lysates using HB-EGF Rabbit mAb (1:1000 diluted). Predicted band size: 23kDa. Observed band size: 23kDa.")
![ELISA: Mouse HB-EGF ELISA Kit (Colorimetric) [NBP2-62763] - Samples were spiked with high concentrations of Mouse HB-EGF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62763_mouse-hb-egf-elisa-kit-colorimetric-132202416254019.jpg "ELISA: Mouse HB-EGF ELISA Kit (Colorimetric) [NBP2-62763] - Samples were spiked with high concentrations of Mouse HB-EGF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse HB-EGF ELISA Kit (Colorimetric) [NBP2-62763] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62763_mouse-hb-egf-elisa-kit-colorimetric-432024916334.jpg)
![Western Blot: HB-EGF Overexpression Lysate (Adult Normal) [NBL1-11459] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HB-EGF.](http://images.novusbio.com/fullsize/HB-EGF-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11459-img0002.jpg "Western Blot: HB-EGF Overexpression Lysate (Adult Normal) [NBL1-11459] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HB-EGF.")