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![Western Blot: Histone H3 [Crotonyl Lys9] Antibody (RM339) [NBP2-77404] - Western Blot using NBP2-77404 against H3K9cr [Crotonyl-Histone H3 (Lys9)]. Anti-Histone H3 and anti-G6PDH were used as controls. A crotonylation inducing metabolite was used to increase the H3K9cr signal. Theoretical molecular weight is ~15 kDa](http://images.novusbio.com/fullsize/Histone-H3-Crotonyl-Lys9-Antibody-RM339-Western-Blot-NBP2-77404-img0001.jpg "Western Blot: Histone H3 [Crotonyl Lys9] Antibody (RM339) [NBP2-77404] - Western Blot using NBP2-77404 against H3K9cr [Crotonyl-Histone H3 (Lys9)]. Anti-Histone H3 and anti-G6PDH were used as controls. A crotonylation inducing metabolite was used to increase the H3K9cr signal. Theoretical molecular weight is ~15 kDa")
![Dot Blot: Histone H3 [Crotonyl Lys9] Antibody (RM339) [NBP2-77404] - A Peptide dot blot showing NBP2-77404 reacts specifically to Histone H3 crotonylated at Lysine 9 (H3K9-Crotonyl), and NBP2-77404's cross-reactivity with different peptides.](http://images.novusbio.com/fullsize/Histone-H3-Crotonyl-Lys9-Antibody-RM339-Dot-Blot-NBP2-77404-img0002.jpg)
![Western Blot: Histone H3 [ac Lys23] Antibody (JE46-39) [NBP3-32428] - Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (NBP3-32428) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500 ng/mL TSA for 4 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 500 ng/mL TSA for 4 hours cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 15 kDa Observed band size: 15/14 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32428) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32428_rabbit-histone-h3-ac-lys23-mab-je46-39-278202415295541.jpg "Western Blot: Histone H3 [ac Lys23] Antibody (JE46-39) [NBP3-32428] - Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (NBP3-32428) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500 ng/mL TSA for 4 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 500 ng/mL TSA for 4 hours cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 15 kDa Observed band size: 15/14 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32428) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys23] Antibody (JE46-39) [NBP3-32428] - Immunocytochemistry analysis of HeLa cells treated with or without 500 ng/mL TSA for 4 hours labeling Histone H3 with Rabbit anti-Histone H3 antibody (NBP3-32428) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Histone H3 antibody (NBP3-32428) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32428_rabbit-histone-h3-ac-lys23-mab-je46-39-2920241192342.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys36] Antibody (C.15200182) [NBP2-59296] - ChIP assays were performed using human HeLa cells, the antibody against H3K36me2 and optimized PCR primer pairs for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a genomic region upstream of the TGM2 gene, used as a positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys36-Antibody-C-15200182-Chromatin-Immunoprecipitation-NBP2-59296-img0002.jpg "Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys36] Antibody (C.15200182) [NBP2-59296] - ChIP assays were performed using human HeLa cells, the antibody against H3K36me2 and optimized PCR primer pairs for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a genomic region upstream of the TGM2 gene, used as a positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).")
![ELISA: Histone H3 [Dimethyl Lys36] Antibody (C.15200182) [NBP2-59296] - Cross reactivity of the antibody directed against H3K36me2. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K36me2. The wells were coated with peptides containing the unmodified H3K36 region as well as the mono-, di- and trimethylated H3K36 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys36-Antibody-C-15200182-ELISA-NBP2-59296-img0001.jpg)
![Western Blot: Histone H3 [Lactyl Lys18] Antibody [NBP3-15600] - Western blot analysis of extracts of MCF7 cells, using Histone H3 antibody (NBP3-15600) at 1:500 dilution.MCF7 cells were treated by Sodium lactate(100mM) for 24h. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5min.](http://images.novusbio.com/fullsize/Histone-H3-[Lactyl-Lys18]-Antibody-Western-Blot-NBP3-15600-img0001.jpg "Western Blot: Histone H3 [Lactyl Lys18] Antibody [NBP3-15600] - Western blot analysis of extracts of MCF7 cells, using Histone H3 antibody (NBP3-15600) at 1:500 dilution.MCF7 cells were treated by Sodium lactate(100mM) for 24h. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5min.")
![Dot Blot: Histone H3 [Lactyl Lys18] Antibody [NBP3-15600] - Dot-blot analysis of all sorts of peptides using Histone H3 antibody (NBP3-15600) at 1:1000 dilution.](http://images.novusbio.com/fullsize/Histone-H3-[Lactyl-Lys18]-Antibody-Dot-Blot-NBP3-15600-img0002.jpg)
![Western Blot: Histone H3 [Monomethyl Arg2] Antibody (3Y5F9) [NBP3-15834] - Western blot analysis of extracts of various cell lines, using Histone H3 antibody (NBP3-15834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Arg2-Antibody-3Y5F9-Western-Blot-NBP3-15834-img0001.jpg "Western Blot: Histone H3 [Monomethyl Arg2] Antibody (3Y5F9) [NBP3-15834] - Western blot analysis of extracts of various cell lines, using Histone H3 antibody (NBP3-15834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Dot Blot: Histone H3 [Monomethyl Arg2] Antibody (3Y5F9) [Histone H3] - Dot-blot analysis of all sorts of peptides using Histone H3 antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/nbp3-15834_rabbit-histone-h3-monomethyl-arg-2-mab-3y5f9-52202517273515.jpg)
![Western Blot: Histone H3 [Dimethyl Lys36] Antibody (RM141) - Azide and BSA Free [NBP3-25987] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM141 at 0. 5 ug/mL, showed a band of histone H3 dimethylated at Lysine 36 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-25987_rabbit-histone-h3-mab-rm141-azide-and-bsa-free-24620241332469.png "Western Blot: Histone H3 [Dimethyl Lys36] Antibody (RM141) - Azide and BSA Free [NBP3-25987] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM141 at 0. 5 ug/mL, showed a band of histone H3 dimethylated at Lysine 36 in HeLa cells.")
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys36] Antibody (RM141) - Azide and BSA Free [NBP3-25987] - RM141 specifically reacts to Histone H3 dimethylated at Lysine 36 (K36me2). No cross reactivity with non-modified Lysine 36 (K36 Ctrl), monomethylated Lysine 36 (K36me1), trimethylated Lysine 36 (K36me3), or other methylations in Histone H3.](http://images.novusbio.com/fullsize/nbp3-25987_rabbit-histone-h3-mab-rm141-azide-and-bsa-free-2462024132613.png)
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys14] Antibody (RM165) - Azide and BSA Free [NBP3-25999] - RM165 specifically reacts to Histone H3 dimethylated at Lysine 14 (K14me2). No cross reactivity with non-modified Lysine 14 (H3k14), monomethylated Lysine 14 (K14me1), trimethylated Lysine 14 (K14me3), or other methylations in Histone H3.](http://images.novusbio.com/fullsize/nbp3-25999_rabbit-histone-h3-mab-rm165-azide-and-bsa-free-2462024124591.png "Multiplex Immunoassay: Histone H3 [Dimethyl Lys14] Antibody (RM165) - Azide and BSA Free [NBP3-25999] - RM165 specifically reacts to Histone H3 dimethylated at Lysine 14 (K14me2). No cross reactivity with non-modified Lysine 14 (H3k14), monomethylated Lysine 14 (K14me1), trimethylated Lysine 14 (K14me3), or other methylations in Histone H3.")
![Western Blot: Histone H3 [Dimethyl Lys14] Antibody (RM165) - Azide and BSA Free [NBP3-25999] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM165 at 0.25 ug/mL, showed a band of histone H3 dimethylated at Lysine 14 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-25999_rabbit-histone-h3-mab-rm165-azide-and-bsa-free-22620242311021.png)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody (RM340) - Azide and BSA Free [NBP3-25983] - SNAP-ChIP TM / qPCR using Clone RM340. Antibody (3 ug at 17 ug/mL) was tested in native ChIP with 3 ug HEK-293 chromatin (~1x10^6 cells). Specificity (left Y-axis; all bars mean +/- SEM from six independent ChIP experiments; note <15% crossreactivity outside H3K4me3) was determined by quantitative real-time PCR (qPCR) for the duplicate DNA barcodes corresponding to each modified nucleosome in the SNAP-ChIP K-MetStat panel (X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded H3K4me3 nucleosome target immunoprecipitated relative to Input.](http://images.novusbio.com/fullsize/nbp3-25983_rabbit-histone-h3-mab-rm340-azide-and-bsa-free-22620242311085.png "Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody (RM340) - Azide and BSA Free [NBP3-25983] - SNAP-ChIP TM / qPCR using Clone RM340. Antibody (3 ug at 17 ug/mL) was tested in native ChIP with 3 ug HEK-293 chromatin (~1x10^6 cells). Specificity (left Y-axis; all bars mean +/- SEM from six independent ChIP experiments; note <15% crossreactivity outside H3K4me3) was determined by quantitative real-time PCR (qPCR) for the duplicate DNA barcodes corresponding to each modified nucleosome in the SNAP-ChIP K-MetStat panel (X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded H3K4me3 nucleosome target immunoprecipitated relative to Input.")
![ELISA: Histone H3 [Trimethyl Lys4] Antibody (RM340) - Azide and BSA Free [NBP3-25983] - ELISA of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with Histone H3 Lysine 4 Modified (H3K4)), using Clone RM340 (10 ug/mL).](http://images.novusbio.com/fullsize/nbp3-25983_rabbit-histone-h3-mab-rm340-azide-and-bsa-free-22620242217441.png)
![ELISA: Histone H3 [Trimethyl Lys9] Antibody (RM389) - Azide and BSA Free [NBP3-25992] - ELISA of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with Histone H3 K4, 9, 27, 36 and H4 K20 Mono-, Di-, and Tri-Methylation), using Clone RM389 (0.5 ug/mL).](http://images.novusbio.com/fullsize/nbp3-25992_rabbit-histone-h3-mab-rm389-azide-and-bsa-free-24620241332430.png "ELISA: Histone H3 [Trimethyl Lys9] Antibody (RM389) - Azide and BSA Free [NBP3-25992] - ELISA of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with Histone H3 K4, 9, 27, 36 and H4 K20 Mono-, Di-, and Tri-Methylation), using Clone RM389 (0.5 ug/mL).")
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (RM389) - Azide and BSA Free [NBP3-25992] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM389 at 0.5 ug/mL, showed a band of histone H3 trimethylated at Lysine 9 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-25992_rabbit-histone-h3-mab-rm389-azide-and-bsa-free-2462024133244.png)