| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26002 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26015 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26005 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25991 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26003 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26018 | |||
![Western Blot: Histone H3 [Trimethyl Lys79] Antibody (RM157) [NBP2-61508] - Western Blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61508 at 0.5 ug/ml, showed a band of Histone H3 trimethylated at Lysine 79 (K79me3) in HeLa cells. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys79-Antibody-RM157-Western-Blot-NBP2-61508-img0004.jpg "Western Blot: Histone H3 [Trimethyl Lys79] Antibody (RM157) [NBP2-61508] - Western Blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61508 at 0.5 ug/ml, showed a band of Histone H3 trimethylated at Lysine 79 (K79me3) in HeLa cells. Observed molecular weight is ~17 kDa.")
![Dot Blot: Histone H3 [Trimethyl Lys79] Antibody (RM157) [NBP2-61508] - A Peptide dot blot shows NBP2-61508 reacts only to Histone H3 trimethyl-Lysine 79 (K79me3). No cross reactivity with nonmodified Lysine 79 (H3K79Ctrl), monomethylated Lysine 79 (K79me1) or dimethylated Lysine 79 (K79me2).](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys79-Antibody-RM157-Dot-Blot-NBP2-61508-img0006.jpg)
![Multiplex Immunoassay: Histone H3 [Trimethyl Lys79] Antibody (RM157) [NBP2-61508] - NBP2-61508 specifically reacts to Histone H3 trimethylated at Lysine 79 (K79me3). No cross reactivity with other methylated lysines in Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys79-Antibody-RM157-Multiplex-Immunoassay-NBP2-61508-img0005.jpg)
![Western Blot: Histone H3 [ac Lys23] Antibody (RM169) [NBP2-61544] - Western Blot of acid extracts of HeLa cells non-treated (1) or treated with sodium butyrate (2), and recombinant Histone H3.3 (3). using NBP2-61544. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys23-Antibody-RM169-Western-Blot-NBP2-61544-img0004.jpg "Western Blot: Histone H3 [ac Lys23] Antibody (RM169) [NBP2-61544] - Western Blot of acid extracts of HeLa cells non-treated (1) or treated with sodium butyrate (2), and recombinant Histone H3.3 (3). using NBP2-61544. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys23] Antibody (RM169) [NBP2-61544] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using NBP2-61544 (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys23-Antibody-RM169-Immunocytochemistry-Immunofluorescence-NBP2-61544-img0003.jpg)
![Multiplex Immunoassay: Histone H3 [ac Lys23] Antibody (RM169) [NBP2-61544] - NBP2-61544 specifically reacts to Histone H3 acetylated at Lysine 23 (K23ac). No cross reactivity with unmodified Lysine 23 (K23 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys23-Antibody-RM169-Multiplex-Immunoassay-NBP2-61544-img0005.jpg)
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys23] Antibody (RM171) [NBP2-61516] - NBP2-61516 specifically reacts to Histone H3 dimethylated at Lysine 23 (K23me2). No cross reactivity with other methylated lysines in Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys23-Antibody-RM171-Multiplex-Immunoassay-NBP2-61516-img0006.jpg "Multiplex Immunoassay: Histone H3 [Dimethyl Lys23] Antibody (RM171) [NBP2-61516] - NBP2-61516 specifically reacts to Histone H3 dimethylated at Lysine 23 (K23me2). No cross reactivity with other methylated lysines in Histone H3.")
![Dot Blot: Histone H3 [Dimethyl Lys23] Antibody (RM171) [NBP2-61516] - A peptide dot blot shows NBP2-61516 reacts only to Histone H3 Dimethyl-Lysine 23 (K23me2). No cross reactivity with nonmodified Lysine 23 (K23Ctrl), monomethylated Lysine 23 (K23me1), or dimethylated Lysine 4 (K4me2).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys23-Antibody-RM171-ELISA-NBP2-61516-img0007.jpg)
![Western Blot: Histone H3 [Dimethyl Lys23] Antibody (RM171) [NBP2-61516] - Recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61516 at 0.5 ug/mL. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys23-Antibody-RM171-Western-Blot-NBP2-61516-img0005.jpg)
![Western Blot: Histone H3 [Monomethyl Lys79] Antibody (RM147) [NBP2-61531] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61531 at 1 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys79-Antibody-RM147-Western-Blot-NBP2-61531-img0006.jpg "Western Blot: Histone H3 [Monomethyl Lys79] Antibody (RM147) [NBP2-61531] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61531 at 1 ug/ml. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys79] Antibody (RM147) [NBP2-61531] - ChIP performed on HeLa cells using H3K79me1 antibody (NBP2-61531). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys79-Antibody-RM147-Chromatin-Immunoprecipitation-NBP2-61531-img0008.jpg)
![Multiplex Immunoassay: Histone H3 [Monomethyl Lys79] Antibody (RM147) [NBP2-61531] - NBP2-61531 specifically reacts to Histone H3 monomethylated at Lysine 79 (K79me1). No cross reactivity with dimethylated Lysine 79 (K79me2) or trimethylated Lysine 79 (K79me3), or other methylations in histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys79-Antibody-RM147-Multiplex-Immunoassay-NBP2-61531-img0007.jpg)
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys18] Antibody (RM168) [NBP2-61514] - NBP2-61514 specifically reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with other methylated lysines in Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys18-Antibody-RM168-Multiplex-Immunoassay-NBP2-61514-img0005.jpg "Multiplex Immunoassay: Histone H3 [Dimethyl Lys18] Antibody (RM168) [NBP2-61514] - NBP2-61514 specifically reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with other methylated lysines in Histone H3.")
![Dot Blot: Histone H3 [Dimethyl Lys18] Antibody (RM168) [NBP2-61514] - A Peptide dot blot shows NBP2-61514 only reacts to Histone. H3 dimethyl-Lysine 18 (K18me2). No cross reactivity with nonmodified Lysine 18 (K18Ctrl), monomethylated. Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2), or dimethylated Lysine 36 (K36me2).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys18-Antibody-RM168-Dot-Blot-NBP2-61514-img0006.jpg)
![Western Blot: Histone H3 [Dimethyl Lys18] Antibody (RM168) [NBP2-61514] - Western Blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61514 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys18-Antibody-RM168-Western-Blot-NBP2-61514-img0004.jpg)
![Western Blot: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H3 (lane 3) using the antibody against H3K79me1. The antibody was diluted 1:200 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys79]-Antibody-Western-Blot-NBP2-59210-img0001.jpg "Western Blot: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H3 (lane 3) using the antibody against H3K79me1. The antibody was diluted 1:200 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - ChIP assays were performed using human HeLa cells, the antibody against H3K79me1 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a region 1 kb upstream of the promoter and the coding region of the active GAPDH gene, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys79]-Antibody-Chromatin-Immunoprecipitation-NBP2-59210-img0003.jpg)
![ELISA: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K79me1. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:11,500.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys79]-Antibody-ELISA-NBP2-59210-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-C-15200184-Immunofluorescence-NBP2-59302-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - ChIP assays were performed using human HeLa cells, the antibody against H3K27ac and optimized PCR primer pairs for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the EIF4A2 and GAPDH genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-C-15200184-Chromatin-Immunoprecipitation-NBP2-59302-img0002.jpg)
![ELISA: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - To test the specificity an ELISA was performed using a serial dilution of the monoclonal antibody against H3K27ac. The wells were coated with peptides containing the unmodified H3K27 region as well as the acetylated H3K27 and the acetylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-C-15200184-ELISA-NBP2-59302-img0005.jpg)
![Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3]-Antibody-Western-Blot-NBP3-12929-img0003.jpg "Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Histone H3 (phospho Thr3) antibody detects Histone H3 (phospho Thr3) protein by western blot analysis. Un-treated (-) and treated (+, 0.1uM Calyculin for 30 minutes + phosphatase inhibitor) HeLa whole cell extracts (2 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) at a dilution of 1:10000.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3]-Antibody-Western-Blot-NBP3-12929-img0001.jpg)
![Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3]-Antibody-Western-Blot-NBP3-12929-img0002.jpg)
![Western Blot: Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free [NBP3-26014] - Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant Histone H3.1 protein, using RM186 at 1 ug/mL, showed a band of Histone H3 unmodified at Lys4.](http://images.novusbio.com/fullsize/nbp3-26014_rabbit-histone-h3-mab-rm186-azide-and-bsa-free-226202422174413.png "Western Blot: Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free [NBP3-26014] - Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant Histone H3.1 protein, using RM186 at 1 ug/mL, showed a band of Histone H3 unmodified at Lys4.")
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free [NBP3-26014] - Immunocytochemical staining of HeLa cells using Clone RM186 (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-26014_rabbit-histone-h3-mab-rm186-azide-and-bsa-free-22620242311013.png)
![Western Blot: Histone H3 [p Ser10] Antibody (RM163) [NBP2-61545] - Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole. Using NBP2-61545, showed a band of Histone H3 phosphorylated at serine 10 in HeLa cells. Observed molecular weight is ~16 kDa](http://images.novusbio.com/fullsize/Histone-H3-p-Ser10-Antibody-RM163-Western-Blot-NBP2-61545-img0003.jpg "Western Blot: Histone H3 [p Ser10] Antibody (RM163) [NBP2-61545] - Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole. Using NBP2-61545, showed a band of Histone H3 phosphorylated at serine 10 in HeLa cells. Observed molecular weight is ~16 kDa")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser10] Antibody (RM163) [NBP2-61545] - Immunocytochemistry of HeLa cells using NBP2-61545 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-p-Ser10-Antibody-RM163-Immunocytochemistry-Immunofluorescence-NBP2-61545-img0002.jpg)