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![Western Blot: Chk2 [p Thr68] Antibody [NBP3-13076] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:1000.](http://images.novusbio.com/fullsize/Chk2-[p-Thr68]-Antibody-Western-Blot-NBP3-13076-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk2 [p Thr68] Antibody [NBP3-13076] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Chk2 [p Thr68] Antibody [NBP3-13076] - Chk2 (phospho Thr68) antibody detects Chk2 (phospho Thr68) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Chk2 (phospho Thr68) stained by Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Chk2-[p-Thr68]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13076-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Chk2 [p Thr68] Antibody [NBP3-13076] - Wild-type (WT) and Chk2 (phospho Thr68) knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Chk2-[p-Thr68]-Antibody-Western-Blot-NBP3-13076-img0003.jpg)
![Western Blot: Chk2 [p Ser516] Antibody [NBP3-16900] - Western blot analysis of extracts of NIH/3T3 cells, using Chk2 pAb (NBP3-16900) at 1:1000 dilution or Chk2 antibody.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Chk2-[p-Ser516]-Antibody-Western-Blot-NBP3-16900-img0001.jpg "Western Blot: Chk2 [p Ser516] Antibody [NBP3-16900] - Western blot analysis of extracts of NIH/3T3 cells, using Chk2 pAb (NBP3-16900) at 1:1000 dilution or Chk2 antibody.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Chk2 [p Thr68] Antibody (JE43-12) [NBP3-32756] - Western blot analysis of Chk2 on different lysates with Rabbit anti-Chk2 antibody (NBP3-32756) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 20uM Etoposide for 2 hours cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 25uM Etoposide for 5 hours cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 25uM Etoposide for 5 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32756) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32756_rabbit-chk2-p-thr68-mab-je43-12-288202412442927.jpg "Western Blot: Chk2 [p Thr68] Antibody (JE43-12) [NBP3-32756] - Western blot analysis of Chk2 on different lysates with Rabbit anti-Chk2 antibody (NBP3-32756) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 20uM Etoposide for 2 hours cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 25uM Etoposide for 5 hours cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 25uM Etoposide for 5 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32756) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Chk2 [p Ser19] Antibody [NBP3-13328] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Ser19) antibody (NBP3-13328) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Chk2-[p-Ser19]-Antibody-Western-Blot-NBP3-13328-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk2 [p Ser19] Antibody [NBP3-13328] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Ser19) antibody (NBP3-13328) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Chk2 Overexpression Lysate (Adult Normal) [NBL1-09147] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Chk2.](http://images.novusbio.com/fullsize/Chk2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09147-img0002.jpg "Western Blot: Chk2 Overexpression Lysate (Adult Normal) [NBL1-09147] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Chk2.")
![Western Blot: Chk2 Overexpression Lysate (Adult Normal) [NBP2-10753] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Chk2.](http://images.novusbio.com/fullsize/Chk2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10753-img0001.jpg "Western Blot: Chk2 Overexpression Lysate (Adult Normal) [NBP2-10753] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Chk2.")