| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4239-SP | |||
2017-T2), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line), as measured by CalceinAM. Adhesion elicited by Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20174). The ND50 is typically 0.05-0.4 µg/mL." alt="Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (Catalog # 2017-T2), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line), as measured by CalceinAM. Adhesion elicited by Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20174). The ND50 is typically 0.05-0.4 µg/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB20174-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08822 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-42998-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50475-0.025ml | |||
![Western Blot: ILT2/CD85j/LILRB1 Antibody [NBP2-15014] - Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/ILT2-CD85j-LILRB1-Antibody-Western-Blot-NBP2-15014-img0002.jpg "Western Blot: ILT2/CD85j/LILRB1 Antibody [NBP2-15014] - Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE; antibody diluted at 1:10000.")
![LILRB1 antibody [N1C1] detects LILRB1 protein by immunofluorescent analysis.</br>Sample: NK-92 cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: LILRB1 stained by LILRB1 antibody [N1C1] (NBP2-15014) diluted at 1:100.</br>Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15014_rabbit-polyclonal-ilt2-cd85j-lilrb1-antibody-immunocytochemistry-immunofluorescence-261120251971823.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LILRB1 antibody [N1C1] (NBP2-15014) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15014_rabbit-polyclonal-ilt2-cd85j-lilrb1-antibody-western-blot-2611202518345214.jpg)
![Flow Cytometry: ILT2/CD85j/LILRB1 Antibody (HP-F1) [NBP1-42998] - Analysis using the Biotin conjugate of NBP1-42998. Staining of normal human peripheral blood cells with 0.125 micrograms of Mouse IgG1 k Isotype Control Biotin (blue histogram) or Anti-Human CD85j (ILT2) Biotin (purple histogram) followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/ILT2-CD85j-LILRB1-Antibody-HP-F1-Flow-Cytometry-NBP1-42998-img0003.jpg "Flow Cytometry: ILT2/CD85j/LILRB1 Antibody (HP-F1) [NBP1-42998] - Analysis using the Biotin conjugate of NBP1-42998. Staining of normal human peripheral blood cells with 0.125 micrograms of Mouse IgG1 k Isotype Control Biotin (blue histogram) or Anti-Human CD85j (ILT2) Biotin (purple histogram) followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.")
![Flow Cytometry: ILT2/CD85j/LILRB1 Antibody (HP-F1) [NBP1-42998] - Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (blue histogram) or Anti-Human CD85j (ILT2) APC (purple histogram). Cells in the monocyte gate were used for analysis.](http://images.novusbio.com/fullsize/ILT2-CD85j-LILRB1-Antibody-HP-F1-Flow-Cytometry-NBP1-42998-img0001.jpg)
![Western Blot: LILRB1 Overexpression Lysate (Adult Normal) [NBP2-07594] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LILRB1.](http://images.novusbio.com/fullsize/LILRB1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07594-img0001.jpg "Western Blot: LILRB1 Overexpression Lysate (Adult Normal) [NBP2-07594] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LILRB1.")
![Western Blot: ILT2/CD85j/LILRB1 Antibody (PSH04-59) [NBP3-32537] - Western blot analysis of ILT2/CD85j/LILRB1 on different lysates with Rabbit anti-ILT2/CD85j/LILRB1 antibody (NBP3-32537) at 1/1,000 dilution.<br/><br/> Lane 1: Raji cell lysate<br/> Lane 2: Daudi cell lysate<br/> Lane 3: THP-1 cell lysate<br/> Lane 4: A431 cell lysate (negative)<br/> Lane 5: K-562 cell lysate (negative)<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 71 kDa<br/> Observed band size: 120 kDa<br/><br/> Exposure time: 1 minute; ECL;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32537) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32537_rabbit-ilt2-cd85j-lilrb1-mab-psh04-59-27820241631625.jpg "Western Blot: ILT2/CD85j/LILRB1 Antibody (PSH04-59) [NBP3-32537] - Western blot analysis of ILT2/CD85j/LILRB1 on different lysates with Rabbit anti-ILT2/CD85j/LILRB1 antibody (NBP3-32537) at 1/1,000 dilution.<br/><br/> Lane 1: Raji cell lysate<br/> Lane 2: Daudi cell lysate<br/> Lane 3: THP-1 cell lysate<br/> Lane 4: A431 cell lysate (negative)<br/> Lane 5: K-562 cell lysate (negative)<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 71 kDa<br/> Observed band size: 120 kDa<br/><br/> Exposure time: 1 minute; ECL;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32537) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
