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Galanin occurs in nerve fibers in most parts of the gastrointestinal tract and in nerve cell bodies in both the submucous and the myenteric plexus. Galanin has multiple effects on gut smooth muscle. Absorption with 10-100mg galanin 1-29 per ml diluted antiserum abolishes the staining, while galanin 1-10, VIP, NPY and Substance P do not.