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DHX16, also known as Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16, is a 119.3 kDa 1,041 amino acid protein and is engaged in pre-mRNA splicing and unwinding RNA as a part of the DEAD box protein family. Current research is being conducted with the protein involving diseases such as adenocarcinoma, ataxia, esophagitis, erythematosus, and malaria. The protein interacts in mRNA processing and spliceosome pathways with other proteins such as GPKOW, SF3B2, COIL, DHX38, and MEGF10.