Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59375-25ug | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-75127 | |||
| Biotin | NBP1-75128 | |||
| DyLight 350 | NBP1-75948 | |||
| DyLight 405 | NBP1-75949 | |||
| DyLight 488 | NBP1-75132 | |||
| DyLight 550 | NBP1-75950 | |||
| DyLight 594 | NBP1-75951 | |||
| DyLight 633 | NBP1-75952 | |||
| DyLight 650 | NBP1-75953 | |||
| FITC | NBP1-75129 | |||
| HRP | NBP1-75130 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-204SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NBP1-06651AFP488 | |||
| Alexa Fluor Plus 555 | NBP1-06651AFP555 | |||
| Alexa Fluor Plus 594 | NBP1-06651AFP594 | |||
| Alexa Fluor Plus 647 | NBP1-06651AFP647 | |||
| Alexa Fluor Plus 680 | NBP1-06651AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00354-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06124-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05970-50ul | |||
![Western Blot: ATP7b Antibody (S62-29) [NBP2-59375] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa Copper Transporting ATPase 2 protein using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (NBP2-59375). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (NBP2-59375) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.](http://images.novusbio.com/fullsize/ATP7b-Antibody-S62-29-Western-Blot-NBP2-59375-img0005.jpg "Western Blot: ATP7b Antibody (S62-29) [NBP2-59375] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa Copper Transporting ATPase 2 protein using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (NBP2-59375). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (NBP2-59375) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.")
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (NBP2-59375). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (NBP2-59375) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Copper Transporting ATPase 2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/ATP7b-Antibody-S62-29-Immunocytochemistry-Immunofluorescence-NBP2-59375-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375] - Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X.](http://images.novusbio.com/fullsize/ATP7b-Antibody-S62-29-Immunocytochemistry-Immunofluorescence-NBP2-59375-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (NBP2-59375). Tissue: NIH 3T3 (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (NBP2-59375) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Copper Transporting ATPase 2 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/ATP7b-Antibody-S62-29-Immunocytochemistry-Immunofluorescence-NBP2-59375-img0007.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75321] - Total cell lysate from human UMUC1. This image was submitted via customer Review. Image from the HRP version of Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-75321-img0004.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75321] - Total cell lysate from human UMUC1. This image was submitted via customer Review. Image from the HRP version of Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).")
![Western Blot: CDK4 Antibody (DCS-31) [NB120-6315] - WB analysis of lysates from human HeLa and SKUT cells using CDK4 antibody (clone DCS-31).](http://images.novusbio.com/fullsize/CDK4-Antibody-DCS-31-Western-Blot-NB120-6315-img0001.jpg "Western Blot: CDK4 Antibody (DCS-31) [NB120-6315] - WB analysis of lysates from human HeLa and SKUT cells using CDK4 antibody (clone DCS-31).")
![Western Blot: RELM beta Antibody [NB200-204] - WB detection of secreted mouse RELM-beta from the media of transfected HEK 293T cells with NB200-204 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/RELM-beta-Antibody-Western-Blot-NB200-204-img0001.jpg "Western Blot: RELM beta Antibody [NB200-204] - WB detection of secreted mouse RELM-beta from the media of transfected HEK 293T cells with NB200-204 antibody at a dilution of 1:500.")
![Immunoprecipitation: COX4NB Antibody [NBP3-00354] - COX4NB was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 2 uL NBP3-00354 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00354, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 24 kDa Observed band size: 24 kDa](http://images.novusbio.com/fullsize/nbp3-00354_rabbit-polyclonal-cox4nb-antibody-235202511444480.png "Immunoprecipitation: COX4NB Antibody [NBP3-00354] - COX4NB was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 2 uL NBP3-00354 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00354, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 24 kDa Observed band size: 24 kDa")
![Immunohistochemistry-Paraffin: COX4NB Antibody [NBP3-00354] - Immunochemical staining of human COX4NB in human placenta with NBP3-00354 (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00354_rabbit-polyclonal-cox4nb-antibody-235202514492366.png)
![Immunohistochemistry-Paraffin: COX4NB Antibody [NBP3-00354] - Immunochemical staining of human COX4NB in human pancreas with NBP3-00354 (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00354_rabbit-polyclonal-cox4nb-antibody-235202521451780.png)
![Western Blot: COX4NB Antibody [NBP3-00354] - NBP3-00354 at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:24 kDa Observed band size:24 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/nbp3-00354_rabbit-polyclonal-cox4nb-antibody-235202514371320.png)
![Immunohistochemistry-Paraffin: COX4NB Antibody [NBP3-00354] - Immunochemical staining of human COX4NB in human skeletal muscle with NBP3-00354 (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00354_rabbit-polyclonal-cox4nb-antibody-23520251628130.png)
![Immunoprecipitation: Casein Kinase 1 alpha Antibody (PSH04-46) [NBP3-32107] - Casein Kinase 1 alpha was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32107 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32107 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32107 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32107 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 11 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32107_rabbit-casein-kinase-1-alpha-mab-psh04-46-2682024158264.jpg "Immunoprecipitation: Casein Kinase 1 alpha Antibody (PSH04-46) [NBP3-32107] - Casein Kinase 1 alpha was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32107 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32107 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32107 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32107 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 11 seconds; ECL")
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 alpha Antibody (PSH04-46) [NBP3-32107] - Immunocytochemistry analysis of HCT 116 cells labeling Casein Kinase 1 alpha with Rabbit anti-Casein Kinase 1 alpha antibody (NBP3-32107) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 1 alpha antibody (NBP3-32107) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32107_rabbit-casein-kinase-1-alpha-mab-psh04-46-29202410484857.jpg)
![Flow Cytometry: Casein Kinase 1 alpha Antibody (PSH04-46) [NBP3-32107] - Flow cytometric analysis of HCT 116 cells labeling Casein Kinase 1 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32107, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32107_rabbit-casein-kinase-1-alpha-mab-psh04-46-29202416372134.jpg)
![Western Blot: Casein Kinase 1 alpha Antibody (PSH04-46) [NBP3-32107] - Western blot analysis of Casein Kinase 1 alpha on different lysates with Rabbit anti-Casein Kinase 1 alpha antibody (NBP3-32107) at 1/2,000 dilution.Lane 1: HCT 116 cell lysate (20 µg/Lane)Lane 2: HeLa cell lysate (20 µg/Lane)Lane 3: HEK-293 cell lysate (20 µg/Lane)Lane 4: MCF7 cell lysate (20 µg/Lane)Lane 5: Jurkat cell lysate (20 µg/Lane)Lane 6: NIH/3T3 cell lysate (20 µg/Lane)Lane 7: RAW264.7 cell lysate (20 µg/Lane)Lane 8: PC-12 cell lysate (20 µg/Lane)Lane 9: Mouse brain tissue lysate (40 µg/Lane)Lane 10: Rat brain tissue lysate (40 µg/Lane)Predicted band size: 39 kDaObserved band size: 34 kDaExposure time: 15 seconds; ECL;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32107_rabbit-casein-kinase-1-alpha-mab-psh04-46-3920248453528.jpg)
![Western Blot: AGTPBP1 Antibody [NBP3-06124] - Anti-AGTPBP1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:138 kDa](http://images.novusbio.com/fullsize/AGTPBP1-Antibody-Western-Blot-NBP3-06124-img0001.jpg "Western Blot: AGTPBP1 Antibody [NBP3-06124] - Anti-AGTPBP1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:138 kDa")
![Immunoprecipitation: AGTPBP1 Antibody [NBP3-06124] -](http://images.novusbio.com/fullsize/AGTPBP1-Antibody-Immunoprecipitation-NBP3-06124-img0002.jpg)
![Immunohistochemistry-Paraffin: AGTPBP1 Antibody - BSA Free [NBP3-06124] - Immunochemical staining of human AGTPBP1 in human skeletal muscle with NBP3-06124 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06124_rabbit-polyclonal-agtpbp1-antibody-235202521451738.png)
![Immunocytochemistry/ Immunofluorescence: AGTPBP1 Antibody - BSA Free [NBP3-06124] - Immunofluorescence staining of AGTPBP1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06124 (dilution ratio 1:200) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-06124_rabbit-polyclonal-agtpbp1-antibody-23520251628197.png)
![Western Blot: VARS Antibody [NBP3-05970] - Lane A: HepG2 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:140 kDaObserved band size:140 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/VARS-Antibody-Western-Blot-NBP3-05970-img0001.jpg "Western Blot: VARS Antibody [NBP3-05970] - Lane A: HepG2 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:140 kDaObserved band size:140 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: VARS Antibody [NBP3-05970] - Staining of human VARS in human brain with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/VARS-Antibody-Immunohistochemistry-Paraffin-NBP3-05970-img0002.jpg)
![Immunoprecipitation: VARS Antibody [NBP3-05970] - Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-VARS rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-VARS rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 140 kDaObserved band size :140 kDa](http://images.novusbio.com/fullsize/VARS-Antibody-Immunoprecipitation-NBP3-05970-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: VARS Antibody [NBP3-05970] - Staining of VARS in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-05970 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-05970_rabbit-polyclonal-vars-antibody-235202512205536.png)