Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27162-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27172-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27148-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9546-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9547-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7286APC | |||
| Biotin | NB7286B | |||
| DyLight 350 | NB7286UV | |||
| DyLight 405 | NB7286V | |||
| DyLight 488 | NB7286G | |||
| DyLight 550 | NB7286R | |||
| DyLight 594 | NB7286DL594 | |||
| DyLight 650 | NB7286C | |||
| DyLight 680 | NB7286FR | |||
| DyLight 755 | NB7286IR | |||
| FITC | NB7288 | |||
| HRP | NB7289 | |||
| Janelia Fluor 525 | NB7286JF525 | |||
| Janelia Fluor 669 | NB7286JF669 | |||
| PE | NB7286PE | |||
| PerCP | NB7286PCP | |||
![Flow Cytometry: SP17 Antibody (1C4C7) [NBP3-27162] - Analysis of SK-OV-3 cells using SP17 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27162_mouse-sp17-mab-1c4c7-bsa-free-10420249141937.jpg%3Fv=1666704905 "Flow Cytometry: SP17 Antibody (1C4C7) [NBP3-27162] - Analysis of SK-OV-3 cells using SP17 mouse mAb (green) and negative control (red).")
![Western Blot: SP17 Antibody (1C4C7) [NBP3-27162] - Analysis using SP17 mAb against human SP17 (AA: 1-152) recombinant protein. (Expected MW is 20.3 kDa)](http://images.novusbio.com/fullsize/nbp3-27162_mouse-sp17-mab-1c4c7-bsa-free-1042024920224.jpg%3Fv=1666704905)
![ELISA: SP17 Antibody (1C4C7) [NBP3-27162] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27162_mouse-sp17-mab-1c4c7-bsa-free-10420249181945.jpg%3Fv=1666704905)
![Western Blot: SP17 Antibody (1C4C7) [NBP3-27162] - Analysis using SP17 mAb against HEK293-6e (1) and SP17 (AA: 1-152)-hIgGFc transfected HEK293-6e (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27162_mouse-sp17-mab-1c4c7-bsa-free-1042024917018.jpg%3Fv=1666704905)
![Immunohistochemistry: SP17 Antibody (1C4C7) [NBP3-27162] - Analysis of paraffin-embedded testi tissues using SP17 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27162_mouse-sp17-mab-1c4c7-bsa-free-1042024921155.jpg%3Fv=1666704905)
![Immunohistochemistry: SP17 Antibody (1C4C7) [NBP3-27162] - Analysis of paraffin-embedded endometrial cancer tissues using SP17 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27162_mouse-sp17-mab-1c4c7-bsa-free-1042024914197.jpg%3Fv=1666704905)
![Immunohistochemistry: FPRL2/FPR3 Antibody (8A6G12) [NBP3-27172] - Analysis of paraffin-embedded rectum cancer tissues using FPRL2/FPR3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27172_mouse-fprl2-fpr3-mab-8a6g12-bsa-free-10420249181949.jpg%3Fv=1666705929 "Immunohistochemistry: FPRL2/FPR3 Antibody (8A6G12) [NBP3-27172] - Analysis of paraffin-embedded rectum cancer tissues using FPRL2/FPR3 mouse mAb with DAB staining.")
![Immunohistochemistry: FPRL2/FPR3 Antibody (8A6G12) [NBP3-27172] - Analysis of paraffin-embedded cervical cancer tissues using FPRL2/FPR3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27172_mouse-fprl2-fpr3-mab-8a6g12-bsa-free-10420249141935.jpg%3Fv=1666705929)
![Western Blot: FPRL2/FPR3 Antibody (8A6G12) [NBP3-27172] - Analysis using FPRL2/FPR3 mAb against human FPRL2/FPR3 recombinant protein. (Expected MW is 38.4 kDa)](http://images.novusbio.com/fullsize/nbp3-27172_mouse-fprl2-fpr3-mab-8a6g12-bsa-free-10420249181923.jpg%3Fv=1666705929)
![ELISA: FPRL2/FPR3 Antibody (8A6G12) [NBP3-27172] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27172_mouse-fprl2-fpr3-mab-8a6g12-bsa-free-1042024916217.jpg%3Fv=1666705929)
![Western Blot: FPRL2/FPR3 Antibody (8A6G12) [NBP3-27172] - Analysis using FPRL2/FPR3 mAb against HEK293-6e (1) and FPRL2/FPR3-hIgGFc transfected HEK293-6e (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27172_mouse-fprl2-fpr3-mab-8a6g12-bsa-free-1042024916218.jpg%3Fv=1666705929)
![Flow Cytometry: FPRL2/FPR3 Antibody (8A6G12) [NBP3-27172] - Analysis of THP-1 cells using FPRL2/FPR3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27172_mouse-fprl2-fpr3-mab-8a6g12-bsa-free-1042024915835.jpg%3Fv=1666705929)
![Western Blot: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis using HBO1 mAb against HEK293 (1) and HBO1 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-104202491704.jpg%3Fv=1666704274 "Western Blot: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis using HBO1 mAb against HEK293 (1) and HBO1 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: HBO1 Antibody (1D9H9) [NBP3-27148] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-1042024916212.jpg%3Fv=1666704274)
![Immunohistochemistry: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis of paraffin-embedded cervical cancer tissues using HBO1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-104202492021.jpg%3Fv=1666704274)
![Western Blot: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis using HBO1 mouse mAb against MOLT4 (1), COS7 (2), F9 (3), HepG2 (4), PC-2 (5), U251 (6), MCF-7 (7), and NIH/3T3 (8) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-1042024916234.jpg%3Fv=1666704274)
![Western Blot: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis using HBO1 mAb against human HBO1 (AA: 1-200) recombinant protein. (Expected MW is 48.1 kDa)](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-1042024917033.jpg%3Fv=1666704274)
![Immunohistochemistry: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis of paraffin-embedded colon cancer tissues using HBO1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-104202491629.jpg%3Fv=1666704274)
![Western Blot: Thrombomodulin/BDCA-3 Antibody (THBD/1591) - Azide and BSA Free [NBP2-54490] - Analysis (A) Recombinant Protein (B) THP1 Cell lysate Using Thrombomodulin/CD141 Monoclonal Antibody (THBD/1591).](http://images.novusbio.com/fullsize/Thrombomodulin-BDCA-3-Antibody-THBD-1591-Azide-and-BSA-Free-Western-Blot-NBP2-54490-img0004.jpg "Western Blot: Thrombomodulin/BDCA-3 Antibody (THBD/1591) - Azide and BSA Free [NBP2-54490] - Analysis (A) Recombinant Protein (B) THP1 Cell lysate Using Thrombomodulin/CD141 Monoclonal Antibody (THBD/1591).")
![Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (THBD/1591) - Azide and BSA Free [NBP2-54490] - Formalin-fixed, paraffin-embedded human Placenta stained with Thrombomodulin/CD141 Monoclonal Antibody (THBD/1591).](http://images.novusbio.com/fullsize/Thrombomodulin-BDCA-3-Antibody-THBD-1591-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54490-img0003.jpg)
![Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (THBD/1591) - Azide and BSA Free [NBP2-54490] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Thrombomodulin Monoclonal/CD141 Antibody (THBD/1591).](http://images.novusbio.com/fullsize/Thrombomodulin-BDCA-3-Antibody-THBD-1591-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54490-img0001.jpg)
![Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (THBD/1591) - Azide and BSA Free [NBP2-54490] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Thrombomodulin/CD141 Monoclonal Antibody (THBD/1591).](http://images.novusbio.com/fullsize/Thrombomodulin-BDCA-3-Antibody-THBD-1591-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54490-img0002.jpg)
![Protein Array: Thrombomodulin/BDCA-3 Antibody (THBD/1591) - Azide and BSA Free [NBP2-54490] - Analysis of Protein Array containing >19,000 full-length human proteins using Thrombomodulin/BDCA-3 Antibody (THBD/1591) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Thrombomodulin-BDCA-3-Antibody-THBD-1591-Azide-and-BSA-Free-Protein-Array-NBP2-54490-img0005.jpg)
![N/A Vitamin D BP [Biotin]](http://images.novusbio.com/fullsize2/Vitamin_D_BP_DY3778_ELISA_2440.jpg "N/A Vitamin D BP [Biotin]")
![ELISA PDGF R beta [Biotin]](http://images.novusbio.com/fullsize2/dyc385-2_human-total-pdgf-r-beta-duoset-ic-elisa-5-plate-elisa-1592020123158.jpg "ELISA PDGF R beta [Biotin]")
![ELISA p38 alpha [Biotin]](http://images.novusbio.com/fullsize2/dyc8691b-2_total-p38-alpha-duoset-ic-elisa-2-plate-elisa-169202013418.jpg "ELISA p38 alpha [Biotin]")