Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Tonsil (double label): CD3 (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-mouse-rabbit-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6200-NB-img0014.jpg "Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Tonsil (double label): CD3 (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).")
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Mesothelioma (double label): M2A Antigen (m), VECTASTAIN Universal Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-mouse-rabbit-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6200-NB-img0001.jpg)
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Mesothelioma (double label): M2A Antigen (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); CD34 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-mouse-rabbit-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6200-NB-img0002.jpg)
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Tonsil (double label): Cyclin A (m), VECTASTAIN Universal ABC-AP Kit, Vector Red substrate (red); CD3 (m), VECTASTAIN Universal Elite ABC Kit, Vector DAB substrate (brown).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-mouse-rabbit-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6200-NB-img0003.jpg)
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Tonsil (double label): Cyclin A (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector NovaRED substrate (red).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-mouse-rabbit-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6200-NB-img0004.jpg)
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Small Bowel (double label): CD45 (m); VECTASTAIN Universal Elite ABC Kit, DAB+Ni substrate (gray/black); Epithelial Specific Antigen (m), VECTASTAIN Universal Elite ABC Kit, DAB substrate (brown).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-mouse-rabbit-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6200-NB-img0005.jpg)
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Tonsil (double label): CD3 (m), VECTASTAIN Universal Elite ABC Kit, Vector DAB substrate (brown); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-mouse-rabbit-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6200-NB-img0006.jpg)
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Tonsil triple stained using the VECTASTAIN Universal Elite ABC Kit: T cells (brown, DAB), B cells (purple, Vector VIP), epithelium (blue/gray, Vector SG).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-mouse-rabbit-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6200-NB-img0007.jpg)
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Small bowel: CD56 (m), VECTASTAIN Universal Elite ABC Kit, ImmPACT DAB (brown) substrate. Hematoxylin QS counterstain (blue).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-mouse-rabbit-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6200-NB-img0008.jpg)
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Kidney (double label): CD34 (m), VECTASTAIN Universal Elite ABC Kit, DAB + Ni substrate (gray/black); Cytokeratin 8/18 (m), VECTASTAIN Universal Elite ABC Kit, DAB.](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-mouse-rabbit-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6200-NB-img0009.jpg)
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Tonsil (triple label): CD3 (m), VECTASTAIN Universal Elite ABC Kit, DAB (brown); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); Multi-cytokeratin (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-mouse-rabbit-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6200-NB-img0010.jpg)
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Colon Carcinoma (double label): S100 (rp), VECTASTAIN Universal ABC-AP Kit, Vector Red substrate (red); Cytokeratin 8/18 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-mouse-rabbit-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6200-NB-img0011.jpg)
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Lymph Node (double label): Ki67 (m), VECTASTAIN Universal ABC-AP Kit, Vector Red Substrate (red); Multi-cytokeratin (m), VECTASTAIN Universal Elite ABC Kit, DAB substrate (brown).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-mouse-rabbit-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6200-NB-img0012.jpg)
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue); CD34 (m), VECTASTAIN Universal Elite ABC Kit, DAB substrate (brown).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-mouse-rabbit-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6200-NB-img0013.jpg)
![Immunohistochemistry: VECTASTAIN(R) ABC anti-Mouse IgG HRP Immunodetection Kit [PK-4002-NB] - Mouse Spinal Cord. Primary antibody: Anti-Myelin Basic Protein Mouse mAb. Detection: VECTASTAIN(R) ABC anti-Mouse IgG HRP Immunodetection Kit and DAB. Image from verified customer review.](http://images.novusbio.com/fullsize/VECTASTAIN-R-ABC-anti-Mouse-IgG-HRP-Immunodetection-Kit-Immunohistochemistry-PK-4002-NB-img0001.jpg "Immunohistochemistry: VECTASTAIN(R) ABC anti-Mouse IgG HRP Immunodetection Kit [PK-4002-NB] - Mouse Spinal Cord. Primary antibody: Anti-Myelin Basic Protein Mouse mAb. Detection: VECTASTAIN(R) ABC anti-Mouse IgG HRP Immunodetection Kit and DAB. Image from verified customer review.")
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-Mouse IgG HRP Immunodetection Kit [PK-6102-NB] - Intestine : Desmin (m), VECTASTAIN Elite ABC Kit, ImmPACT DAB (brown) substrate. Cytokeratin (m), VECTASTAIN Elite ABC Kit, Vector VIP (purple) substrate.](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-Mouse-IgG-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6102-NB-img0004.jpg "Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-Mouse IgG HRP Immunodetection Kit [PK-6102-NB] - Intestine : Desmin (m), VECTASTAIN Elite ABC Kit, ImmPACT DAB (brown) substrate. Cytokeratin (m), VECTASTAIN Elite ABC Kit, Vector VIP (purple) substrate.")
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-Mouse IgG HRP Immunodetection Kit [PK-6102-NB] - Breast Cancer: CD34 antigen stained using VECTASTAIN Elite ABC Kit and Vector DAB (brown) substrate. Hematoxylin QS (blue) counterstain.](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-Mouse-IgG-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6102-NB-img0001.jpg)
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-Mouse IgG HRP Immunodetection Kit [PK-6102-NB] - Breast Cancer: Estrogen receptor stained using VECTASTAIN Elite ABC Kit and Vector DAB (brown) substrate. Hematoxylin QS (blue) counterstain.](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-Mouse-IgG-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6102-NB-img0002.jpg)
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-Mouse IgG HRP Immunodetection Kit [PK-6102-NB] - Breast Cancer: c-erbB2 oncoprotein stained using VECTASTAIN Elite ABC Kit and Vector DAB (brown) substrate. Hematoxylin QS (blue) counterstain.](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-Mouse-IgG-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6102-NB-img0003.jpg)
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-Rabbit IgG HRP Immunodetection Kit [PK-6101-NB] - Small Bowel: Ki67 antigen stained using VECTASTAIN Elite ABC Kit and Vector DAB (brown) substrate. Hematoxylin QS (blue) counterstain.](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-Rabbit-IgG-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6101-NB-img0002.jpg "Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-Rabbit IgG HRP Immunodetection Kit [PK-6101-NB] - Small Bowel: Ki67 antigen stained using VECTASTAIN Elite ABC Kit and Vector DAB (brown) substrate. Hematoxylin QS (blue) counterstain.")
![Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-Rabbit IgG HRP Immunodetection Kit [PK-6101-NB] - Breast Cancer: p53 protein stained using VECTASTAIN Elite ABC Kit and Vector DAB (brown) substrate. Hematoxylin QS (blue) counterstain.](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-anti-Rabbit-IgG-HRP-Immunodetection-Kit-Immunohistochemistry-PK-6101-NB-img0001.jpg)