VECTASTAIN® ELITE® ABC anti-mouse/rabbit HRP Immunodetection Kit

Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Tonsil (double label): CD3 (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).

Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Mesothelioma (double label): M2A Antigen (m), VECTASTAIN Universal Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue).

Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Mesothelioma (double label): M2A Antigen (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); CD34 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).

Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Tonsil (double label): Cyclin A (m), VECTASTAIN Universal ABC-AP Kit, Vector Red substrate (red); CD3 (m), VECTASTAIN Universal Elite ABC Kit, Vector DAB substrate (brown).

Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Tonsil (double label): Cyclin A (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector NovaRED substrate (red).

Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Small Bowel (double label): CD45 (m); VECTASTAIN Universal Elite ABC Kit, DAB+Ni substrate (gray/black); Epithelial Specific Antigen (m), VECTASTAIN Universal Elite ABC Kit, DAB substrate (brown).

Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Tonsil (double label): CD3 (m), VECTASTAIN Universal Elite ABC Kit, Vector DAB substrate (brown); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).

Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Tonsil triple stained using the VECTASTAIN Universal Elite ABC Kit: T cells (brown, DAB), B cells (purple, Vector VIP), epithelium (blue/gray, Vector SG).

Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Small bowel: CD56 (m), VECTASTAIN Universal Elite ABC Kit, ImmPACT DAB (brown) substrate. Hematoxylin QS counterstain (blue).

Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Kidney (double label): CD34 (m), VECTASTAIN Universal Elite ABC Kit, DAB + Ni substrate (gray/black); Cytokeratin 8/18 (m), VECTASTAIN Universal Elite ABC Kit, DAB.

Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Tonsil (triple label): CD3 (m), VECTASTAIN Universal Elite ABC Kit, DAB (brown); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); Multi-cytokeratin (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).

Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Colon Carcinoma (double label): S100 (rp), VECTASTAIN Universal ABC-AP Kit, Vector Red substrate (red); Cytokeratin 8/18 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).

Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Lymph Node (double label): Ki67 (m), VECTASTAIN Universal ABC-AP Kit, Vector Red Substrate (red); Multi-cytokeratin (m), VECTASTAIN Universal Elite ABC Kit, DAB substrate (brown).

Immunohistochemistry: VECTASTAIN(R) ELITE(R) ABC anti-mouse/rabbit HRP Immunodetection Kit [PK-6200-NB] - Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue); CD34 (m), VECTASTAIN Universal Elite ABC Kit, DAB substrate (brown).

• Reactivity: Mu, Rb
• Applications: WB, ELISA, Func, ICC/IF, IHC, ELISA

VECTASTAIN® ELITE® ABC anti-mouse/rabbit HRP Immunodetection Kit Summary

• Description: Features:
• Advanced avidin/biotin technology: The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target
• Highest sensitivity, low background: The VECTASTAIN® Elite® ABC system is the most sensitive avidin/biotin-based peroxidase system. The Elite® ABC series is approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background.
• Cost effective: Higher sensitivity leads to lower cost per slide
• Available without ("Standard" kit) or with biotinylated species-specific or universal secondary antibodies
• Available in ready-to-use formats: Prediluted, stabilized working solutions of Elite® ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN® Elite® ABC Kit reagents.
• Kit Type: HRP Immunodetection Kit

Applications/Dilutions

• Dilutions:
  • ELISA
  • Enzyme-linked Immunosorbent Spot
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • In-situ Hybridization
  • Western Blot
• Application Notes: Kit contains sufficeint reagents to stain approximately 500-1000 sections..

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

With more than 50,000 citations to its credit, the VECTASTAIN(R) ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.
Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN(R) ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.