| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38370-25ul | |||
HAF109). A specific band was detected for Peroxiredoxin 5 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of human, mouse, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5724) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 5 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5724-SP | |||
HAF017). A specific band was detected for Peroxiredoxin 5 at approximately 17 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2." alt="Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, A431 human epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Peroxiredoxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3774) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Peroxiredoxin 5 at approximately 17 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3774-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89862-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38371-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22124-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59388-50ul | |||
![Immunoprecipitation: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Peroxiredoxin 5 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32740 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32740 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br/><br/> Lane 1: HeLa cell lysate (input)<br/> Lane 2: NBP3-32740 IP in HeLa cell lysate<br/> Lane 3: Rabbit IgG instead of NBP3-32740 in HeLa cell lysate<br/><br/> Blocking/Dilution buffer: 5% NFDM/TBST<br/> Exposure time: 43 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-26820241582610.jpg "Immunoprecipitation: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Peroxiredoxin 5 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32740 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32740 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br/><br/> Lane 1: HeLa cell lysate (input)<br/> Lane 2: NBP3-32740 IP in HeLa cell lysate<br/> Lane 3: Rabbit IgG instead of NBP3-32740 in HeLa cell lysate<br/><br/> Blocking/Dilution buffer: 5% NFDM/TBST<br/> Exposure time: 43 seconds; ECL")
![Immunohistochemistry: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Peroxiredoxin 5 antibody (NBP3-32740) at 1/10,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32740) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-298202414262615.jpg)
![Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Immunocytochemistry analysis of HeLa cells labeling Peroxiredoxin 5 with Rabbit anti-Peroxiredoxin 5 antibody (NBP3-32740) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Peroxiredoxin 5 antibody (NBP3-32740) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-292024111484.jpg)
![Flow Cytometry: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Flow cytometric analysis of HeLa cells labeling Peroxiredoxin 5.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32740, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-29202416372132.jpg)
![Western Blot: Peroxiredoxin 5 Antibody [NBP2-38370] - Analysis using Anti-PRDX5 antibody NBP2-38370 (A) shows similar pattern to independent antibody NBP2-38371 (B).](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Western-Blot-NBP2-38370-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Peroxiredoxin 5 Antibody [NBP2-38370] - Analysis using Anti-PRDX5 antibody NBP2-38370 (A) shows similar pattern to independent antibody NBP2-38371 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody [NBP2-38370] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38370-img0005.jpg)
NL001) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for 
NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (mitochondria). View our protocol for ![Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Western-Blot-NBP2-89862-img0002.jpg "Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa")
![Immunoprecipitation: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate 2 uL anti-PRDX5 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-PRDX5 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 22 kDa Observed band size: 22 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Immunoprecipitation-NBP2-89862-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: PRDX5 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:15 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Knockout-Validated-NBP2-89862-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Staining of PRDX5 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-89862 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89862_rabbit-monoclonal-peroxiredoxin-5-antibody-002-235202514492332.png)
![Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38371] - Staining in human fallopian tube and liver tissues using anti-PRDX5 antibody. Corresponding PRDX5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38371-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38371] - Staining in human fallopian tube and liver tissues using anti-PRDX5 antibody. Corresponding PRDX5 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Peroxiredoxin 5 Antibody [NBP2-38371] - Analysis using Anti-PRDX5 antibody NBP2-38371 (A) shows similar pattern to independent antibody NBP2-38370 (B).](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Western-Blot-NBP2-38371-img0007.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38371] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38371-img0004.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38371] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38371-img0005.jpg)
![Western Blot: Peroxiredoxin 5 Antibody (SR1264) [NBP3-22124] - Western blot analysis using NBP3-22124 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22124_rabbit-peroxiredoxin-5-mab-sr1264-572023153122.jpg "Western Blot: Peroxiredoxin 5 Antibody (SR1264) [NBP3-22124] - Western blot analysis using NBP3-22124 in HepG2 cell lysate.")
![Western Blot: Peroxiredoxin 5 Antibody (6A10) [NBP2-59388] - Lane 1: Recombinant PRDX5 protein, Lane 2: MCF-7 cell lysates, Lane 3: A549 cell lysates, Lane 4: HeLa cell lysates, Lane 5: 293T cell lysates, Lane 6: Jurkat cell lysates](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-6A10-Western-Blot-NBP2-59388-img0001.jpg "Western Blot: Peroxiredoxin 5 Antibody (6A10) [NBP2-59388] - Lane 1: Recombinant PRDX5 protein, Lane 2: MCF-7 cell lysates, Lane 3: A549 cell lysates, Lane 4: HeLa cell lysates, Lane 5: 293T cell lysates, Lane 6: Jurkat cell lysates")
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody (6A10) [NBP2-59388] - Analysis of PRDX5 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-6A10-Immunocytochemistry-Immunofluorescence-NBP2-59388-img0002.jpg)
![Immunoprecipitation:[NBP3-29520]-Detection of human PRDX5 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PRDX5 antibody used for IP at 6 µg per reaction. PRDX5 was also immunoprecipitated by rabbit anti-PRDX5 antibody. For blotting immunoprecipitated PRDX5, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/nbp3-29520_rabbit-peroxiredoxin-5-pab-231202512101683.jpg "Immunoprecipitation:[NBP3-29520]-Detection of human PRDX5 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PRDX5 antibody used for IP at 6 µg per reaction. PRDX5 was also immunoprecipitated by rabbit anti-PRDX5 antibody. For blotting immunoprecipitated PRDX5, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Western Blot: Peroxiredoxin 5 Antibody [NBP1-58348] - Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Western-Blot-NBP1-58348-img0004.jpg "Western Blot: Peroxiredoxin 5 Antibody [NBP1-58348] - Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0 ug/ml")
![Western Blot: Peroxiredoxin 5 Antibody [NBP1-58348] - Titration: 0.2-1 ug/ml, Positive Control: Human Lung.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Western-Blot-NBP1-58348-img0003.jpg)
![Western Blot: Peroxiredoxin 5 Antibody (5E10D3) [NBP3-16300] - Western blot analysis of extracts of various cell lines, using Peroxiredoxin 5 (PRDX5) Rabbit mAb (NBP3-16300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10148 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-5E10D3-Western-Blot-NBP3-16300-img0001.jpg "Western Blot: Peroxiredoxin 5 Antibody (5E10D3) [NBP3-16300] - Western blot analysis of extracts of various cell lines, using Peroxiredoxin 5 (PRDX5) Rabbit mAb (NBP3-16300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10148 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
