T Cell Exhaustion Marker (Flow) Antibody Packs: Novus Biologicals

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NBP3-12864

T Cell Exhaustion Marker (Flow) Antibody Pack

9 Vials

NBP3-12864

Flow Cytometry: T Cell Exhaustion Marker (Flow) - Human peripheral blood lymphocytes were stained with Mouse Anti-Human TIGIT Monoclonal Antibody (Catalog # MAB7898) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog# F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog# FAB2408P). Quadrant markers were set based on control antibody staining (Catalog# MAB0041).

Flow Cytometry: T Cell Exhaustion Marker (Flow) - Mouse splenocytes were stained with Rat Anti-Mouse CD161/NK1.1 APC-conjugated Monoclonal Antibody (Catalog#: FAB7614A) and either (A) Rabbit Anti-Mouse KLRG1 Monoclonal Antibody (Catalog# MAB6944or (B) Normal Rabbit IgG Control (Catalog# AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# F0110) at 10 uL/10^6 cells.

Flow Cytometry: T Cell Exhaustion Marker (Flow) - Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 5 ng/mL PHA for 2 days or (B) untreated, were stained with Rabbit Anti-Human PD-1 Monoclonal Antibody (Catalog # MAB10863) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# F0111) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog# FAB100P). Quadrant markers were set based on control antibody staining (Catalog# MAB1050).

Flow Cytometry: T Cell Exhaustion Marker (Flow) - NS0 mouse myeloma cell line transfected with human CTLA-4 and eGFP was stained with (A) Rabbit Anti-Human CTLA-4 Monoclonal Antibody (Catalog # MAB3253) or (B) Normal Rabbit IgG Control Antibody (Catalog# MAB1050) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog# F0111).

Flow Cytometry: T Cell Exhaustion Marker (Flow) - A cell surface stain was performed on U-937 cells with VISTA/B7-H5/PD-1H (730823) antibody MAB71263PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

Reactivity:

Hu

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Reactivity: Hu

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