Top 4 reasons: why use CRISPR-Cas9 antibodies and how?

1. Verification of the success of transfection

Why- If the CRISPR-Cas9 transfection is not successful, it would not be relevant to relate the observations from transfected cells to the expected outcome of gene editing experiment.

CRISPR-associated system 9 (CAS9) – a useful tool in gene editing studies

CRISPR-associated system 9 (CAS9)

CAS9 is a novel DNA-cutting enzyme that is the main component of an intrinsic DNA editing system used by bacteria to kill attacking viruses. Clustered regularly interspaced short palindromic repeats (CRISPR) are distinct features of most bacterial genomes, and thought to be involved in resistance to bacteriophages. CRISPR is a primitive immune system of sorts that determines resistance specificity, as published by the Danisco Corporation in Science1.