Stem Cell Marker Antibodies now validated for FACS

Several stem cell markers from the Novus Biologicals antibody catalog were recently validated for use in fluorescent-activated cell sorting (FACS), including CRIPTO1, SOX2, PODXL, and GPR49/LGR5. In particular, the following  stem cell marker antibodies are now suitable for FACS: Cripto1 Antibody (NB100-1597), Cripto1 Antibody (NB100-1598) , SOX2 Antibody (NB110-37235), PODXL Antibody (NB110-41503), GPR49/LGR5 Antibody (NBP1-28904). 

FACS, a type of flow chemistry, is a useful tool for analyzing how many cells express a specific protein and how much of this protein is produced. In this process, cell-specific surface antigens are labeled with fluorescent antibodies specific to particular antigens. A suspension of these cells is passed through a stream in the form of droplets, with one cell per droplet. After applying an electrical charge, cells can be separated by charge depending on which antibody is bound to the surface of the antigen.

The five stem cell markers mentioned above have also been tested for many other techniques, such as Western Blot, Immunohistochemistry, Immunofluorescence, and Immunocytochemistry. However, FACS provides the advantage of analyzing the rate of expression of a particular protein using antigen-specific antibodies.

FACS images for these stem cell marker antibodies are now available on each antibody datasheet. The rabbit polyclonal anti-Cripto1 antibodies (NB100-1597 and NB100-1598) were both used for FACS analysis of NTERA-2 cells at a 1:50 dilution (view image here for NB100-1597 and here for NB100-1598). The other three antibodies recently validated for FACS analysis were used for analyses under the same conditions (view image here for SOX2 antibody, here for PODXL antibody, and here for GPR49/LGR5 antibody). For more information, or to test these antibodies, explore our antibody database.


For more information on stem cell markers with FACS application, please contact the Novus Technical Support Team by calling 303-730-1950 or via e-mail at

Release Date: 
Wednesday, June 9, 2010 - 06:00