DNA Newsletter - 8th Edition

Thursday, March 24, 2011 - 00:00
In this DNA edition, read about a spotlight on PARP, PARP antibodies, PARP inhibitor news, and new IP machine.


DNA Newsletter

In This Edition

Spotlight on PARP

Poly (ADP-ribose) polymerase (PARP) is a 17 member protein family, involved in DNA repair and programmed cell death. PARP proteins detect single strand DNA breaks (SSB) and initiate the mechanism responsible for base excision repair. PARP is immediately activated in response to chemical, metabolic or radiation-induced SSBs. Upon SSB detection, PARP binds the DNA and synthesizes a poly (ADP-ribose) chain (PAR) at the damaged site. The PAR chain then signals the initiation of DNA repair by other enzymes.

This base excision repair mechanism carries a high energy cost, as extensive PARP activation can exhaust cellular NAD+ stores and lead to progressive ATP depletion. Therefore, PARP inactivation occurs in cells with extensive DNA damage, where too much energy would be required to make the necessary repairs. In such cases, a programmed cell death pathway is initiated to conserve energy for other, less damaged cells in the tissue.

Featured Products

PARP (C-2-10) Antibody
Catalog # NB100-111
Species: Hu, Mk, Rt
Applications: ELISA, ICC, WB

Cleaved p25 PARP (E51) Antibody
Catalog # NB110-57320
Species: Hu, Mu
Applications: FACS, ICC,

Cleaved p85 PARP (Y34) Antibody
Catalog # NB110-57321
Species: Hu, Rt
Applications: FACS, ICC,

WB Image of PARP
IF Image of Cleaved p25 PARP
FACS Image of Cleaved p85 PARP
WB analysis of
whole cell extracts from (1)
HL-60 lysates and (2) HL-60 lysates induced to undergo apoptosis using NB100-111.
IF analysis of Hela cell
using NB110-57320.
FACS analysis of apoptotic
and non-apoptotic Jurkat
cells using NB110-57321.


PARP Inhibitors In the News

PARP Inhibitors And Breast Cancer Treatment
PARP Inhibitor Drugs


Precipitor™ – a machine for high throughput IP & protein purification

IHC Image of LKB1

Are you wasting time on IP? The Precipitorâ„¢ system is
an automated magnetic bead platform for high-throughput precipitation and purification of proteins. Improve the efficiency of your lab today!

Learn more HERE or contact us at
precipitor@novusbio.com to set up an online demo.


We look forward to providing you with quality research tools,

Amy Chamberlain-Torres

Product Marketing Manager