![Western Blot: Phospho-Tyrosine Antibody (13F9) [NB600-907] - Lane 1: phosphorylated EGFR in an A431 cell lysate. Lane 2: total protein in the unstimulated A431 cells lysate after coomassie staining. Load: 10 ug per lane. Primary antibody: phosphotyrosine antibody at 1:1000 for 1 hr at RT. Secondary antibody: IRDye800 Conjugated Goat-a-Mouse IgG [H&L] diluted 1:2,500 for 30' at room temperature. Block: 5% BLOTTO overnight at 4C. Other band(s): phosphotyrosine splice variants and isoforms.](http://images.novusbio.com/fullsize/Phospho-Tyrosine-Antibody-13F9-Western-Blot-NB600-907-img0006.jpg "Western Blot: Phospho-Tyrosine Antibody (13F9) [NB600-907] - Lane 1: phosphorylated EGFR in an A431 cell lysate. Lane 2: total protein in the unstimulated A431 cells lysate after coomassie staining. Load: 10 ug per lane. Primary antibody: phosphotyrosine antibody at 1:1000 for 1 hr at RT. Secondary antibody: IRDye800 Conjugated Goat-a-Mouse IgG [H&L] diluted 1:2,500 for 30' at room temperature. Block: 5% BLOTTO overnight at 4C. Other band(s): phosphotyrosine splice variants and isoforms.")
| Immunogen | This monoclonal antibody was produced after repeated immunizations of balb/c mice with Phospho-Tyrosine conjugated KLH. |
| Specificity | Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphoserine or phosphothreonine. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | This monoclonal antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphothreonine. The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated - carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation. Phosphorylation of tyrosine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation. |
|
| Reviewed Applications |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Ascites |
| Preservative | 0.01% Sodium Azide |
| Purity | Unpurified |
| Images | Ratings | Applications | Species | Date | Details | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
|
reviewed by:
Verified Customer |
WB | Human | 01/26/2010 |
Summary
|
Secondary Antibodies |
Isotype Controls |
Research Areas for Phospho-Tyrosine Antibody (NB600-907)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![ELISA: Phospho-Tyrosine Antibody (13F9) [NB600-907] - ELISA results of Mab anti-phosphotyrosine antibody tested against BSA conjugates of pT, pY and pS. Each well was coated with 0.1ug of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution.](http://images.novusbio.com/fullsize/Phospho-Tyrosine-Antibody-13F9-ELISA-NB600-907-img0003.jpg "ELISA: Phospho-Tyrosine Antibody (13F9) [NB600-907] - ELISA results of Mab anti-phosphotyrosine antibody tested against BSA conjugates of pT, pY and pS. Each well was coated with 0.1ug of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution.")