Western Blot Protocol for RPE65 Antibody (NB100-355)

Western Blot

1. Bovine RPE membrane (5ug) and recombinant human RPE65 (~20ug ) expressed in COS7 cells were loaded on a NuPAGE Novex Bis-Tris 10%gel along with SeeBlue Plus2 marker.

2. Run in NuPAGE MOPS electrophoresis buffer (Invitrogen).

3 Samples were probed with NB 100-355 RPE65 antibody (1:5,000 ) followed by alkaline phosphatase-conjugated goat anti-mouse IgG incubation.

4. Visualized by NBT/BCIP substrate.