Western Blot Protocol for Nucleophosmin Antibody (NB110-61646)

Western Blot

1. Run 50 ug of cytoplasmic fraction into each lane of an 12% SDS-PAGE.

2. Block the membrane.

3. Dilute the primary anti-mNPM1 (NB110-61646) in 1% NFDM and incubate for 2 hours at room temperature.

4. Wash the membrane.

5. Dilute the sedondary anti-rabbit IgG, conjugated to HRP in 1% NFDM and incubate for 1 hour at room temperature.

6. Develop the blot.