Western Blot Protocol for GAPDH Antibody (NB300-328)

Western Blot

1. Resolve proteins on a 10% SDS-PAGE.

2. Transfer to nitrocellulose.

3. Block in 5% NFDM overnight at 4C.

4. Dilute anti-GAPDH (NB 300-328) 1:5,000 in blocking buffer and incubate for 2 hours at RT.

5. Dilute secondary goat anti-mouse IgG (whole molecule) conjugated to HRP (as per manufacturer's guidelines) and incubate for 1 hour at RT.

6. Develop membrane using ECL reagent.