Western Blot Protocol for CITED4 Antibody (NB110-41572)

Western Blot

1. Run a 10% reducing gel. Boil samples in running buffer.

2. Block the nitrocellulose membrane in 15% milk/5% Tween-20 for 1 hour at room temperature.

3. Dilute the primary anti-CITED4 (NB110-41572) antibody in media and incubate the membrane for 1 hour at room temperature.

4. Probe the membrane with an anti-mouse IgG, HRP conjugated secondary antibody, as per the manufacturer's specifications.

5. Develop the blot using an ECL kit.