Western Blot Protocol for CD34 Antibody (NB500-608): https://www.novusbio.com/products/cd34-antibody-4h11-apg_nb500-608
Use Kg-1a human leukemia cell lysate as a positive control and JURKAT human leukemia T-cell line as a negative control.
Sample preparation: Resuspend approx. 50 million cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Application note: non-reducing conditions.