Home » SDS-Page protocol for Sorbitol Dehydrogenase Recombinant Protein (NBP1-44460)

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SDS-Page protocol for Sorbitol Dehydrogenase Recombinant Protein (NBP1-44460)

SDS-PAGE (NBP1-44460):
1. Prepare a 1.5 ml assay buffer. The final concentrations are 78 mM Triethanolamine, 183 mM D-Fructose, 0.2 mM beta-nicotinamide dinucleotide and 0.003 % (w/v) bovine serum albumin.

2. Add 50 ul of recombinant SORD protein in various concentrations (1 ug, 2 ug, 5 ug) in assay buffer.

3. Mix by inversion and record the decrease at A340nm for 5 minutes.