Home » SDS-Page protocol for Catalase Recombinant Protein (NBP1-98907)

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SDS-Page protocol for Catalase Recombinant Protein (NBP1-98907)

SDS-PAGE (NBP1-98907):
1. Prepare an assay buffer with the following concentrations: 50 mM Potassium Phosphate (pH 8.0) and 0.15 % H2O2.

2. Load 150ul assay buffer into each well

3. Add 50ul of recombinant CAT protein with 0.25ug, 0.125ug to each well.

4. Record the decrease in A260nm for 5 minutes at 25C using UV plate.