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1. Prepare a 200ul reaction mix into a suitable container: The final concentrations are 10mM Tris (pH7.5), 50mM NaCl, 2mM DTT, 1mM MnCl2, 10mM pNPP.
2. Pre-warm reaction mix in heat block at 37C.
3. Add recombinant DUSP3 protein solution with various concentrations (1ug, 2ug, 3ug) in 200ul reaction buffer.
4. Mix by inversion and incubate at 37C for 10minutes.
5. Stop the reaction by adding the 800ul of 0.1N NaOH and record the absorbance at 405nm.
