Nuclear Extract and Cytoplasmic Fraction Preparation protocol for GPCR GPR40 Antibody (NLS1595):
Procedure Guide for NLS 1595 - G Protein-Coupled Receptor GPR40
Antibody
Immunohistochemistry
1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4-mm sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene alpha 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol alpha 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol alpha 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol alpha 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water alpha 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.
**Do not allow the tissues to dry at any time during the staining procedure**
12. Begin the immunostaining by applying a universal protein block alpha 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody alpha 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T alpha 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary alpha 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T alpha 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin alpha 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T alpha 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate alpha 30 minutes @ RT.
20. Rinse the slide in distilled water alpha 1 minute @ RT.
**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**
21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol alpha 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol alpha 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol alpha 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene alpha 1 minute each @ RT.
25. Apply cover slip.
