Product Handling - Assay Method (NBP2-29397)

Materials:
Dissolve 5 mg of Z-IETD-FMK in DMSO to get appropriate concentration:
765 ml DMSO = 10 mM
383 ml DMSO = 20 mM
1530 ml DMSO = 5 mM

Method:
Add 2 ml of above stock solutions to 1 ml of culture medium containing cells to give final DMSO concentration of 0.2%. Levels of DMSO above this may cause some cellular toxicity thus masking the effect of the ICE-protease inhibitors. 2 ml of 10 mM stock in 1 ml medium = 20 mM final Z-IETD-FMK concentration.