Product Handling - Assay Method (NBP2-29395)

Dissolve 1mg of BOC-D-FMK in DMSO to get appropriate concentration:
190 ul DMSO = 20mM
381 ul DMSO = 10 mM
762 ul DMSO = 5 mM

Add 2 ul of above stock solutions to 1 ml of culture medium containing cells to give final DMSO concentration of 0.2%. Levels of DMSO above this may cause some cellular toxicity thus masking the effect of the ICE-protease inhibitors. 2 ul of 10 mM stock in 1 ml medium = 20 uM final BOC-D-FMK concentration.