Product Handling - Assay Method (NBP2-29392)

Dissolve 1mg of Z-Val-Ala-Asp(OMe)-FMK in DMSO to get appropriate concentration:
107 ul DMSO = 20mM
214 ul DMSO = 10 mM
428 ul DMSO = 5 mM

Add 2 ul of above stock solutions to 1 ml of culture medium containing cells to give final DMSO concentration of
0.2%. Levels of DMSO above this may cause some cellular toxicity thus masking the effect of the ICE-protease