Product Handling - Assay Method (NBP2-29398)

Materials:
Dissolve 5 mg of Z-LEHD-FMK in DMSO to get appropriate concentration:
310 ul DMSO = 20 mM
620 ul DMSO = 10 mM
1240 ul DMSO = 5 mM

Method:
Add 2 ul of above stock solutions to 1 ml of culture medium containing cells to give final DMSO concentration of
0.2%. Levels of DMSO above this may cause some cellular toxicity thus masking the effect of the ICE-protease
inhibitors. 2 ul of 10 mM stock in 1 ml medium = 20 uM final Z-LEHD-FMK concentration.