Immunohistochemistry protocol for Carbonic Anhydrase IX Antibody (NB100-417)

Immunohistochemistry Procedure
1. Using paraffin-embedded tissue sections, dewax and rehydrate the tissue, as per standard protocol.
2. Block the slides with peroxidase for 5 minutes.
3. Incubate slides with primary antibody [NB 100-417], 1:1,000 diluted in 10% normal serum, for 30 minutes at room temperature (RT).
4. Wash slides for 5 minutes.
5. Incubate slides with secondary antibody [envision, DAKO] for 30 minutes at RT.
6. Wash slides for 5 minutes.
7. Incubate slides with DAB chromagen for 6 minutes.
8. Counterstain slides with Hematoxylin. NOTE: Antigen retrieval was not used.