Flow Cytometry protocol specific for CCR5 Antibody (NB100-531)

Flow Cytometry

1. CEM.NKR.CCR5 cells were stained with 25 ug/ml anti-CCR5 (NB 100-531) or with preimmune rabbit IgG for 60 minutes on ice.

2. This was followed by extensive washing and staining with a FITC-conjugated goat anti-rabbit secondary antibody.

3. Flow cytometry was performed and analyzed with Cell Quest Software on FACSCalibur (BD Biosciences).