Flow Cytometry (NBP1-30426):
Positive control: Human T cells stimulated with anti-CD3 (MEM-57) antibody (1 mg/ml)
Sample preparation:
At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS
+ 0.1% BSA for 20 min. on ice.
Wash with PBS and fix with 2% formaldehyde, 30 min on ice.
Wash with PBS and incubate in PBS + 0,1% saponine, 5 min. RT.
Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice.
Incubate with fluorescence-labeled APA1/1 antibody (1-5 mg/ml) in PBS + 1% BSA + 0.03%
saponine in dark, 20 min. RT.
Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS.
