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Flow Cytometry protocol for Anti-Mouse Ig (H+L) Comp-Bead 3 Population (5.5 um) Kit (NBP3-00497)

Shake vigorously or vortex before use. Sonication will help to increase the number of singles. Use for routine quality control of mouse antibody conjugates used in flow cytometry. Add 1 drop from each bottle to a centrifuge tube, followed by the addition of the conjugate being evaluated. After exposure to appropriate mouse antibody conjugates, three different fluorescent peaks shall be resolved by flow cytometry.