AEC Chromogen/Substrate Kit Protocol (NB900-79773):
Staining Procedure
1. Once sections have been incubated with peroxidase, wash sections with wash buffer.
2. Wipe slides to remove excess buffer. Add enough drops of AEC Chromogen/Substrate to cover tissue sections.
3. Incubate for 5-15 minutes at room temperature.
4. For best results, observe reaction under a microscope for signal development.
5. Once desired signal to noise ratio is achieved, stop reaction by washing slides in DI H2O.
