| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88153-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37247-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05378-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26692-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21555-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-129SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26693-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-130SS | |||
![Immunocytochemistry/Immunofluorescence: Cyclin E1 Antibody [NBP1-88153] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88153-img0006.jpg "Immunocytochemistry/Immunofluorescence: Cyclin E1 Antibody [NBP1-88153] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mouse mAb against Hela (1), K562 (2), NIH/3T3 (3), C6 (4), MCF-7 (5), Jurkat (6), A431 (7) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-5F8C5-Western-Blot-NBP2-37247-img0004.jpg "Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mouse mAb against Hela (1), K562 (2), NIH/3T3 (3), C6 (4), MCF-7 (5), Jurkat (6), A431 (7) cell lysate.")
![Flow Cytometry: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Flow cytometric analysis of K652 cells using CCNE1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-5F8C5-Flow-Cytometry-NBP2-37247-img0005.jpg)
![Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mAb against human CCNE1 (AA: 307-410) recombinant protein. (Expected MW is 37.5 kDa)](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-5F8C5-Western-Blot-NBP2-37247-img0002.jpg)
![Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mAb against HEK293 (1) and CCNE1 (AA: 307-410)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-5F8C5-Western-Blot-NBP2-37247-img0003.jpg)
![ELISA: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-5F8C5-ELISA-NBP2-37247-img0001.jpg)
![Western Blot: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (NBP3-05378). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/Cyclin-E1-[p-Thr395]-Antibody-Western-Blot-NBP3-05378-img0002.jpg "Western Blot: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (NBP3-05378). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of C6 cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-7220251245389.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of U2OS cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-72202512432038.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of NIH/3T3 cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-72202512432011.jpg)
![Immunohistochemistry: Cyclin E1 Antibody (3A5) [NBP3-26692] - Image of Cyclin E1 Antibody (3A5) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26692_rabbit-cyclin-e1-mab-3a5-262202414471591.jpg "Immunohistochemistry: Cyclin E1 Antibody (3A5) [NBP3-26692] - Image of Cyclin E1 Antibody (3A5) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Cyclin E1 Antibody (3A5) [NBP3-26692] - Image of Cyclin E1 Antibody (3A5) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26692_rabbit-cyclin-e1-mab-3a5-262202415253332.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin E1 Antibody (3A5) [NBP3-26692] - Staining of Hela Cells with Cyclin E1 Antibody (3A5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26692_rabbit-cyclin-e1-mab-3a5-282202412493621.jpg)
![Western Blot: Cyclin E1 [p Thr 77] Antibody (SR1828) [NBP3-21555] - Western blot analysis using NBP3-21555 in (1) JAR cell treated with Lambda Phosphatase lysate; (2) JAR cell lysate.](http://images.novusbio.com/fullsize/nbp3-21555_rabbit-cyclin-e1-p-thr-77-mab-sr1828-57202315123414.jpg "<b>Biological Strategies Validation. </b>Western Blot: Cyclin E1 [p Thr 77] Antibody (SR1828) [NBP3-21555] - Western blot analysis using NBP3-21555 in (1) JAR cell treated with Lambda Phosphatase lysate; (2) JAR cell lysate.")
![Western Blot: Cyclin E Antibody [NB500-129] - Western Blot analysis of Cyclin E1 on transfected 293T cells at 1:500 dilution.](http://images.novusbio.com/fullsize/Cyclin-E-Antibody-Western-Blot-NB500-129-img0001.jpg "Western Blot: Cyclin E Antibody [NB500-129] - Western Blot analysis of Cyclin E1 on transfected 293T cells at 1:500 dilution.")
![Immunocytochemistry/Immunofluorescence: Cyclin E1 Antibody (9H12) [NBP3-26693] - Staining of Hela Cells with Cyclin E1 Antibody (9H12) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26693_rabbit-cyclin-e1-mab-9h12-282202412475134.jpg "Immunocytochemistry/Immunofluorescence: Cyclin E1 Antibody (9H12) [NBP3-26693] - Staining of Hela Cells with Cyclin E1 Antibody (9H12) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")
![Immunoprecipitation:Cyclin E2 AntibodyNBP3-29686] -Detection of human CCNE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCNE2 antibody NBP3-29686 (lot NBP3-29686-1) used for IP at 6 µg per reaction. CCNE2 was also immunoprecipitated by rabbit anti-CCNE2 antibody. For blotting immunoprecipitated CCNE2, NBP3-29686 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29686_rabbit-cyclin-e2-pab-301220241952587.jpg "Immunoprecipitation:Cyclin E2 AntibodyNBP3-29686] -Detection of human CCNE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCNE2 antibody NBP3-29686 (lot NBP3-29686-1) used for IP at 6 µg per reaction. CCNE2 was also immunoprecipitated by rabbit anti-CCNE2 antibody. For blotting immunoprecipitated CCNE2, NBP3-29686 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: Cyclin E2 Antibody [NBP1-77755] - shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown).](http://images.novusbio.com/fullsize/Cyclin-E2-Antibody-Western-Blot-NBP1-77755-img0001.jpg "Western Blot: Cyclin E2 Antibody [NBP1-77755] - shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown).")
![Western Blot: Cyclin E2 Antibody [NB500-130] - Analysis of Cyclin E2 in CT10 cell line using this antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Cyclin-E2-Antibody-Western-Blot-NB500-130-img0002.jpg "Western Blot: Cyclin E2 Antibody [NB500-130] - Analysis of Cyclin E2 in CT10 cell line using this antibody at 1:500 dilution.")
![Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody [NBP2-16054] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D1 protein stained by Cyclin D1 antibody [N1C3] diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16054-img0007.jpg "Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody [NBP2-16054] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D1 protein stained by Cyclin D1 antibody [N1C3] diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Scale bar = 10 um.")
![Immunoprecipitation: Cyclin D1 Antibody [NBP2-16054] - Immunoprecipitation of Cyclin D1 protein from MCF-7 whole cell extracts using 5 ug of Cyclin D1 antibody [N1C3]. Western blot analysis was performed using Cyclin D1 antibody [N1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Immunoprecipitation-NBP2-16054-img0006.jpg)
![ELISA: Cyclin D1 Antibody [NBP2-16054] - Detection of recombinant full-length Cyclin D1 protein using as capture antibody at concentration of 5 ug/mL and as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-ELISA-NBP2-16054-img0014.jpg)
![Western Blot: Cyclin D1 Antibody [NBP2-16054] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (NBP2-16054) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-910202420122096.jpg)
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-16054] - Cyclin D1 antibody detects Cyclin D1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded cat liver.Cyclin D1 stained by Cyclin D1 antibody (NBP2-16054) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-910202420104464.jpg)
![Western Blot: Cyclin D1 Antibody [NBP2-16054] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (NBP2-16054) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-91020242063359.jpg)
![Western Blot: Cyclin D1 Antibody [NBP2-16054] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (NBP2-16054) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-91020242075916.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclin D1 Antibody [NBP2-16054] - Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-1610202419414560.jpg)
![Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (NBP2-16054) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-western-blot-26112025182002.jpg)