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Read our scientific technical answers to your top 3 frequently asked questions (FAQs) on CRISPR-Cas9:
- CRISPR-Cas9: What's the Buzz About?
- Why Use CRISPR-Cas9 Antibodies?
- Any Publications citing Novus’ CRISPR-Cas9 Antibodies?
CRISPR-Cas9: What's the Buzz About?
CRISPR-Cas9 is an RNA-guided DNA endonuclease enzyme (mwt: 158.4 kDa), which is now widely used in molecular biology for the induction of double strand breaks in DNA. CRISPR-Cas9 technology can be used to induce insertion or deletion mutations, specific sequence replacements or insertions, and large deletions or genomic rearrangements at any desired genomic location. The genes encoding CRISPR-Cas are essential to the bacterial adaptive immune system, enabling bacteria to mount an immune response against invading genetic material. The best characterized CRISPR-associated nucleases are the Cas9 proteins from Streptococcus pyogenes (S. pyogenes).
Why Use CRISPR-Cas9 Antibodies?
Antibodies serve as great tool when working with Cas9 transfectants, and the top four reasons for using CRISPR-Cas9 antibodies are given below:
i. Transfection Efficiency
Verifying the success of CRISPR-Cas9 transfection is the primary reason to use CRISPR-Cas9 antibodies. Comparing the expression of Cas9 in transfected versus mock transfected cells is important to assess the efficiency of transfection. With the help of CRISPR-Cas9 antibodies, one can use Western blot and/or immunostaining to verify transfection efficiency.