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![Western Blot: c-Rel Antibody [NB600-474] - All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.](http://images.novusbio.com/fullsize/c-Rel-Antibody-Western-Blot-NB600-474-img0005.jpg "Western Blot: c-Rel Antibody [NB600-474] - All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.")
![Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.](http://images.novusbio.com/fullsize/nb600-474_rabbit-polyclonal-c-rel-antibody-255202313292313.jpg)
![Immunohistochemistry: N myc interactor Antibody [NB100-763] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NMI used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/N-myc-interactor-Antibody-Immunohistochemistry-NB100-763-img0002.jpg "Immunohistochemistry: N myc interactor Antibody [NB100-763] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NMI used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Immunoprecipitation: N myc interactor Antibody [NB100-763] - Detection of human NMI/N myc interactor by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NMI antibody NB100-763 (lot 2) used for IP at 6 ug per reaction. NMI was also immunoprecipitated by rabbit anti-NMI antibody NB100-763-1. For blotting immunoprecipitated NMI, NB100-763 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/N-myc-interactor-Antibody-Immunoprecipitation-NB100-763-img0003.jpg)
![Western Blot: Nbs1 Antibody [NB100-60648] - Detection of mouse Nbs1 by western blot. Samples: Whole cell lysate (50 ug) from L1210 and CT26.WT cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60648] (lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Observed Molecular Weight at ~95 kDa.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Western-Blot-NB100-60648-img0003.jpg "Western Blot: Nbs1 Antibody [NB100-60648] - Detection of mouse Nbs1 by western blot. Samples: Whole cell lysate (50 ug) from L1210 and CT26.WT cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60648] (lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Observed Molecular Weight at ~95 kDa.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Nbs1 Antibody [NB100-60648] - Detection of mouse Nbs1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from L1210 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60648] used for IP at 3 ug per reaction. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibody from Company B. For blotting immunoprecipitated NBS1, NB100-60648 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Band detected at ~95 kDa.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Immunoprecipitation-NB100-60648-img0004.jpg)
![ELISA: Human SOD1/Cu-Zn SOD ELISA Kit (Colorimetric) [NBP2-75920] - Standard curve](http://images.novusbio.com/fullsize/Human-SOD1-Cu-Zn-SOD-ELISA-Kit-Colorimetric-ELISA-NBP2-75920-img0001.jpg "ELISA: Human SOD1/Cu-Zn SOD ELISA Kit (Colorimetric) [NBP2-75920] - Standard curve")
![Immunohistochemistry: HIST1H3A [Monomethyl Lys9] Antibody (13E1) [NBP3-26135] - Image of HIST1H3A [Monomethyl Lys9] Antibody (13E1) diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.06% DAB.](http://images.novusbio.com/fullsize/nbp3-26135_rabbit-hist1h3a-monomethyl-lys-9-mab-13e1-262202414471558.jpg "Immunohistochemistry: HIST1H3A [Monomethyl Lys9] Antibody (13E1) [NBP3-26135] - Image of HIST1H3A [Monomethyl Lys9] Antibody (13E1) diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.06% DAB.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A [Monomethyl Lys9] Antibody (13E1) [NBP3-26135] - Staining of A549 with HIST1H3A [Monomethyl Lys9] Antibody (13E1) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 489-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26135_rabbit-hist1h3a-monomethyl-lys-9-mab-13e1-282202412493612.jpg)
![Flow Cytometry: HIST1H3A [Monomethyl Lys9] Antibody (13E1) [NBP3-26135] - Overlay Peak curve showing A549 cells stained with HIST1H3A [Monomethyl Lys9] Antibody (13E1) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26135_rabbit-hist1h3a-monomethyl-lys-9-mab-13e1-282202414183677.jpg)
![Immunohistochemistry: PARP Antibody (7C11) [NBP3-26438] - Image of PARP Antibody (7C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26438_rabbit-parp-mab-7c11-262202415355711.jpg "Immunohistochemistry: PARP Antibody (7C11) [NBP3-26438] - Image of PARP Antibody (7C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PARP Antibody (7C11) [NBP3-26438] - Image of PARP Antibody (7C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26438_rabbit-parp-mab-7c11-272202485637.jpg)
![Western Blot: PARP Antibody (7C11) [NBP3-26438] - Positive Western Blot detected in: K562 whole cell lysate. All lanes: PARP Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114 kDa Observed band size: 114 kDa](http://images.novusbio.com/fullsize/nbp3-26438_rabbit-parp-mab-7c11-282202411472110.jpg)