| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98571-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90261-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89507-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97168-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97262-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52639-0.025mg | |||
![Western Blot: Chk1 [p Ser280] Antibody (JE47-66) [NBP3-32754] - Western blot analysis of Chk1 on different lysates with Rabbit anti-Chk1 antibody (NBP3-32754) at 1/1,000 dilution. Lane 1: 293T whole cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: 293T treated with 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32754) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32754_rabbit-chk1-p-ser280-mab-je47-66-288202412442937.jpg "Western Blot: Chk1 [p Ser280] Antibody (JE47-66) [NBP3-32754] - Western blot analysis of Chk1 on different lysates with Rabbit anti-Chk1 antibody (NBP3-32754) at 1/1,000 dilution. Lane 1: 293T whole cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: 293T treated with 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32754) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: PRP19 Antibody [NBP2-98571] - Anti-PRP19 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/PRP19-Antibody-Western-Blot-NBP2-98571-img0003.jpg "Western Blot: PRP19 Antibody [NBP2-98571] - Anti-PRP19 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP2-98571] - Immunochemical staining of human PRP19 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP2-98571-img0002.jpg)
![Immunohistochemistry: BRCA1 Antibody (A7E3-R) [NBP3-32090] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-BRCA1 antibody (NBP3-32090) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32090) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32090_mouse-brca1-mab-a7e3-r-298202414304150.jpg "Immunohistochemistry: BRCA1 Antibody (A7E3-R) [NBP3-32090] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-BRCA1 antibody (NBP3-32090) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32090) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: ING5 Antibody (119) [NBP2-90261] - Anti-ING5 rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/ING5-Antibody-119-Western-Blot-NBP2-90261-img0002.jpg "Western Blot: ING5 Antibody (119) [NBP2-90261] - Anti-ING5 rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: ING5 Antibody (119) [NBP2-90261] - ING5 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-ING5 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ING5 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 32 kDa](http://images.novusbio.com/fullsize/ING5-Antibody-119-Immunoprecipitation-NBP2-90261-img0001.jpg)
![Western Blot: Bcl-xL Antibody (016) [NBP2-89507] - Lane A: HepG2 Whole Cell Lysate Lane B: K562 Whole Cell LysateLane C: NIH-3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDa Observed band size:30 kDa](http://images.novusbio.com/fullsize/Bcl-xL-Antibody-016-Western-Blot-NBP2-89507-img0002.jpg "Western Blot: Bcl-xL Antibody (016) [NBP2-89507] - Lane A: HepG2 Whole Cell Lysate Lane B: K562 Whole Cell LysateLane C: NIH-3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDa Observed band size:30 kDa")
![Immunoprecipitation: Bcl-xL Antibody (016) [NBP2-89507] - Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg K562 Whole Cell Lysate1 uL anti-BCL2L1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-BCL2L1 rabbit monoclonal antibody,at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 26 kDa Observed band size: 26 kDa](http://images.novusbio.com/fullsize/Bcl-xL-Antibody-016-Immunoprecipitation-NBP2-89507-img0001.jpg)
![Western Blot: TTC11 Antibody [NBP2-97168] - Anti-TTC11 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lane C: HeLa Whole Cell Lysate. Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 15 kDa](http://images.novusbio.com/fullsize/TTC11-Antibody-Western-Blot-NBP2-97168-img0003.jpg "Western Blot: TTC11 Antibody [NBP2-97168] - Anti-TTC11 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lane C: HeLa Whole Cell Lysate. Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 15 kDa")
![Immunohistochemistry-Paraffin: TTC11 Antibody [NBP2-97168] - Immunochemical staining of human TTC11 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/TTC11-Antibody-Immunohistochemistry-Paraffin-NBP2-97168-img0002.jpg)
![Immunohistochemistry-Paraffin: Bmf Antibody [NBP2-97262] - Immunochemical staining of human Bmf in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Bmf-Antibody-Immunohistochemistry-Paraffin-NBP2-97262-img0002.jpg "Immunohistochemistry-Paraffin: Bmf Antibody [NBP2-97262] - Immunochemical staining of human Bmf in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunocytochemistry/Immunofluorescence: Bmf Antibody [NBP2-97262] - Immunofluorescence staining of Bmf in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97262 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97262_rabbit-polyclonal-bmf-antibody-23520251135416.png)
![Western Blot: TSC2 [p Thr1462] Antibody (JE43-81) [NBP3-32813] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32813) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32813) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32813_rabbit-tsc2-p-thr1462-mab-je43-81-288202412461624.jpg "Western Blot: TSC2 [p Thr1462] Antibody (JE43-81) [NBP3-32813] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32813) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32813) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (143) [NBP2-89212] - Immunochemical staining of human p63/TP73L in human breast carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-143-Immunohistochemistry-Paraffin-NBP2-89212-img0003.jpg "Immunohistochemistry-Paraffin: p63/TP73L Antibody (143) [NBP2-89212] - Immunochemical staining of human p63/TP73L in human breast carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (143) [NBP2-89212] - Staining of human P63 in human prostatic carcinoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-143-Immunohistochemistry-Paraffin-NBP2-89212-img0001.jpg)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (143) [NBP2-89212] - Staining of human P63 in human prostate with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-143-Immunohistochemistry-Paraffin-NBP2-89212-img0002.jpg)
![Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32790_rabbit-pras40-p-thr246-mab-je53-94-288202412442948.jpg "Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: ErbB4/Her4 Antibody (JE59-43) [NBP3-32314] - Western blot analysis of ErbB4/Her4 on different lysates with Rabbit anti-ErbB4/Her4 antibody (NBP3-32314) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 147 kDa Observed band size: 180 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32314) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32314_rabbit-erbb4-her4-mab-je59-43-27820241631641.jpg "Western Blot: ErbB4/Her4 Antibody (JE59-43) [NBP3-32314] - Western blot analysis of ErbB4/Her4 on different lysates with Rabbit anti-ErbB4/Her4 antibody (NBP3-32314) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 147 kDa Observed band size: 180 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32314) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (4D5-8 (trastuzumab)) - Chimeric [NBP2-52639] - Immunohistochemical Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rabbit-chimeric version of 4D5-8 (trastuzumab) (NBP2-52639). Primary antibody incubation with NBP2-52639 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 ug/ml.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-4D5-8-trastuzumab-Chimeric-Immunohistochemistry-Paraffin-NBP2-52639-img0001.jpg "Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (4D5-8 (trastuzumab)) - Chimeric [NBP2-52639] - Immunohistochemical Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rabbit-chimeric version of 4D5-8 (trastuzumab) (NBP2-52639). Primary antibody incubation with NBP2-52639 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 ug/ml.")