| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26614-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-48011-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26431-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26387-50ul | |||
![Immunohistochemistry: DNMT1 Antibody (1A9) [NBP3-26614] - Image of DNMT1 Antibody (1A9) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26614_rabbit-dnmt1-mab-1a9-262202415475617.jpg "Immunohistochemistry: DNMT1 Antibody (1A9) [NBP3-26614] - Image of DNMT1 Antibody (1A9) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunocytochemistry/Immunofluorescence: DNMT1 Antibody (1A9) [NBP3-26614] - Staining of HepG2 Cells with DNMT1 Antibody (1A9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26614_rabbit-dnmt1-mab-1a9-282202412475138.jpg)
![Immunohistochemistry-Paraffin: EGR1 Antibody (C11) [NBP3-41149] - DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 20ug/ml NBP3-41149; Second Ab: 2ug/mLÂ HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41149_mouse-egr1-mab-c11-6122024212745.jpg "Immunohistochemistry-Paraffin: EGR1 Antibody (C11) [NBP3-41149] - DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 20ug/ml NBP3-41149; Second Ab: 2ug/mLÂ HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: EGR1 Antibody (C11) [NBP3-41149] - DAB staining on IHC-P; Samples: Human Prostate Tissue; Primary Ab: 20ug/ml NBP3-41149; Second Ab: 2ug/mLÂ HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41149_mouse-egr1-mab-c11-612202421121323.jpg)
![Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073] - Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody NBP2-32073 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Western blot for total KAP-1 using a different affinity purified rabbit anti-KAP-1 antibody at 0.4 ug/ml with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Western-Blot-NBP2-32073-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073] - Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody NBP2-32073 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Western blot for total KAP-1 using a different affinity purified rabbit anti-KAP-1 antibody at 0.4 ug/ml with an exposure time of 3 seconds.")
![<b>Independent Antibodies Validation. </b>Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073] - Detection of mouse Phospho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from TCMK-1 cells treated with etoposide (+) and/or Lambda PP (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32073 used for IP at 6 ug per reaction. For blotting immunoprecipitated Phospho KAP-1 (S824), NBP2-32073 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-KAP-1 antibody A304-145A.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Immunoprecipitation-NBP2-32073-img0002.jpg)
![Western Blot: KAP1 Antibody [NBP2-32072] - Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-), NIH3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32072 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAP1-Antibody-Western-Blot-NBP2-32072-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: KAP1 Antibody [NBP2-32072] - Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-), NIH3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32072 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: KAP1 Antibody [NBP2-32072] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32072 used for IP at 6 ug/mg lysate. KAP-1 was also immunoprecipitated by rabbit anti-KAP-1 antibody NBP2-32072. For blotting immunoprecipitated KAP-1, NBP2-32072 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunoprecipitation-NBP2-32072-img0003.jpg)
![Western Blot: ATF2 [p Thr71] Antibody (4F2) [NBP3-26431] - Positive Western Blot detected in 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate (treated with Calyculin A or EGF). All lanes: ATF2 [p Thr71] Antibody at 1.015ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 70 KDa Observed band size: 70 KDa](http://images.novusbio.com/fullsize/nbp3-26431_rabbit-atf2-p-thr-71-mab-4f2-28220241152514.jpg "Western Blot: ATF2 [p Thr71] Antibody (4F2) [NBP3-26431] - Positive Western Blot detected in 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate (treated with Calyculin A or EGF). All lanes: ATF2 [p Thr71] Antibody at 1.015ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 70 KDa Observed band size: 70 KDa")
![Immunocytochemistry/Immunofluorescence: ATF2 [p Thr71] Antibody (4F2) [NBP3-26431] - Staining of A549 cells(treated with 100mM EGF for 20min) with ATF2 [p Thr71] Antibody (4F2) at 1:63, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26431_rabbit-atf2-p-thr-71-mab-4f2-28220241323615.jpg)
![Immunohistochemistry: STAT5A [p Tyr694] Antibody (3F1) [NBP3-26387] - Image of STAT5A [p Tyr694] Antibody (3F1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26387_rabbit-stat5a-p-tyr-694-mab-3f1-262202414471572.jpg "Immunohistochemistry: STAT5A [p Tyr694] Antibody (3F1) [NBP3-26387] - Image of STAT5A [p Tyr694] Antibody (3F1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: STAT5A [p Tyr694] Antibody (3F1) [NBP3-26387] - Positive Western Blot detected in HepG2 whole cell lysate (treated with EGF or not). All lanes: STAT5A [p Tyr694] Antibody at 1.025ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 90 KDa Observed band size: 90 KDa](http://images.novusbio.com/fullsize/nbp3-26387_rabbit-stat5a-p-tyr-694-mab-3f1-282202411442631.jpg)
![Western Blot: CSN7b Antibody [NB100-461] - Detection of Human CSN7b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSN7b antibody NB100-461 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CSN7b-Antibody-Western-Blot-NB100-461-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: CSN7b Antibody [NB100-461] - Detection of Human CSN7b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSN7b antibody NB100-461 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: CSN7b Antibody [NB100-461] - Whole cell lysate (50 ug - E; 10 ug - T) from HEK293T cells with (T) or without (E) with a CSN7b expression construct. Antibody used at the indicated concentrations.](http://images.novusbio.com/fullsize/CSN7b-Antibody-Western-Blot-NB100-461-img0003.jpg)