| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99335-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99858-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98812-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98832-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99049-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89187-50ul | |||
![Western Blot: PKA C alpha Antibody [NBP2-99335] - Anti-PKA C alpha rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 44 kDa](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-Western-Blot-NBP2-99335-img0004.jpg "Western Blot: PKA C alpha Antibody [NBP2-99335] - Anti-PKA C alpha rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 44 kDa")
![Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-99335] - Immunochemical staining of human PKA C alpha in human testis with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-99335-img0003.jpg)
![Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-99335] - Immunochemical staining of human PKA C alpha in human colon carcinoma with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-99335-img0002.jpg)
![Western Blot: Protein Kinase D2 Antibody [NBP2-99858] - Anti-Protein Kinase D2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 113 kDa](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NBP2-99858-img0004.jpg "Western Blot: Protein Kinase D2 Antibody [NBP2-99858] - Anti-Protein Kinase D2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 113 kDa")
![Immunoprecipitation: Protein Kinase D2 Antibody [NBP2-99858] - Protein Kinase D2 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate Lane B: 0.5 mg HepG2 Whole Cell Lysate Lane C: 0.5 mg MCF-7 Whole Cell Lysate 4 ul anti-Protein Kinase D2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Protein Kinase D2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size: 100 kDa](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunoprecipitation-NBP2-99858-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Kinase D2 Antibody [NBP2-99858] - Immunofluorescence staining of Protein Kinase D2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99858 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-99858_rabbit-polyclonal-protein-kinase-d2-antibody-235202521525037.png)
![Immunoprecipitation: DYRK1A Antibody [NBP3-30799] - Immunoprecipitation ofDyrk from 293T transfected cells. Immunoprecipitation with- 1) control Ab, 2)Anti-MNP 2 ug/ml, 3)Novus Biologicals' anti-Dyrk 2ug/ml, 4) Novus Biologicals'-Dyrk 10 ug/ml, 5)competitor’s anti-Dyrk Ab 10 ug/ml, Western blot performed with Novus Biologicals' anti-Dyrk Ab at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp3-30799_sheep-dyrk1a-pab-811202420592172.jpg "Immunoprecipitation: DYRK1A Antibody [NBP3-30799] - Immunoprecipitation ofDyrk from 293T transfected cells. Immunoprecipitation with- 1) control Ab, 2)Anti-MNP 2 ug/ml, 3)Novus Biologicals' anti-Dyrk 2ug/ml, 4) Novus Biologicals'-Dyrk 10 ug/ml, 5)competitor’s anti-Dyrk Ab 10 ug/ml, Western blot performed with Novus Biologicals' anti-Dyrk Ab at 2 ug/ml.")
![Western Blot: MuSK Antibody (PSH03-51) [NBP3-32620] - Western blot analysis of MuSK on different lysates with Rabbit anti-MuSK antibody (NBP3-32620) at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse skeletal muscle tissue lysate Lane 4: Mouse hippocampus tissue lysate Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate Lane 7: Rat hippocampus tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32620) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32620_rabbit-musk-mab-psh03-51-278202416331283.jpg "Western Blot: MuSK Antibody (PSH03-51) [NBP3-32620] - Western blot analysis of MuSK on different lysates with Rabbit anti-MuSK antibody (NBP3-32620) at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse skeletal muscle tissue lysate Lane 4: Mouse hippocampus tissue lysate Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate Lane 7: Rat hippocampus tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32620) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MuSK Antibody (PSH03-51) [NBP3-32620] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-MuSK antibody (NBP3-32620) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32620) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32620_rabbit-musk-mab-psh03-51-298202414333641.jpg)
![Western Blot: ZAP70 Antibody (A1B5-R) [NBP3-33110] - Western blot analysis of ZAP70 on different lysates with Mouse anti-ZAP70 antibody (NBP3-33110) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Ramos cell lysate (negative) Lane 3: Mouse spleen tissue lysate Lane 4: Mouse thymus tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat thymus tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33110) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33110_mouse-zap70-mab-a1b5-r-288202414191541.jpg "Western Blot: ZAP70 Antibody (A1B5-R) [NBP3-33110] - Western blot analysis of ZAP70 on different lysates with Mouse anti-ZAP70 antibody (NBP3-33110) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Ramos cell lysate (negative) Lane 3: Mouse spleen tissue lysate Lane 4: Mouse thymus tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat thymus tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33110) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ZAP70 Antibody (A1B5-R) [NBP3-33110] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-ZAP70 antibody (NBP3-33110) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33110) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33110_mouse-zap70-mab-a1b5-r-29820241435257.jpg)
![Immunohistochemistry-Paraffin: JNK2 Antibody [NBP2-98812] - Immunochemical staining of human JNK2 in human pancreas with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/JNK2-Antibody-Immunohistochemistry-Paraffin-NBP2-98812-img0003.jpg "Immunohistochemistry-Paraffin: JNK2 Antibody [NBP2-98812] - Immunochemical staining of human JNK2 in human pancreas with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: JNK2 Antibody [NBP2-98812] - Immunochemical staining of human JNK2 in human lung cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/JNK2-Antibody-Immunohistochemistry-Paraffin-NBP2-98812-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: JNK2 Antibody [NBP2-98812] - Immunofluorescence staining of JNK2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98812 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98812_rabbit-polyclonal-jnk2-antibody-235202511545667.png)
![Immunohistochemistry-Paraffin: TTK Antibody [NBP2-98832] - Staining of human TTK in human lung cancer with rabbit polyclonal antibody (1:1000).](http://images.novusbio.com/fullsize/TTK-Antibody-Immunohistochemistry-Paraffin-NBP2-98832-img0002.jpg "Immunohistochemistry-Paraffin: TTK Antibody [NBP2-98832] - Staining of human TTK in human lung cancer with rabbit polyclonal antibody (1:1000).")
![Immunohistochemistry-Paraffin: TTK Antibody [NBP2-98832] - Staining of human RPS27 in human lung cancer with NBP2-98832 (1:1000).](http://images.novusbio.com/fullsize/nbp2-98832_rabbit-polyclonal-ttk-antibody-235202511444413.png)
![Immunocytochemistry/Immunofluorescence: TTK Antibody [NBP2-98832] - Immunofluorescence staining of TTK in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98832 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98832_rabbit-polyclonal-ttk-antibody-23520252135167.png)
![Immunohistochemistry: IKK alpha Antibody [NBP2-99049] - Immunochemical staining of human IKK in human colon carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-Immunohistochemistry-NBP2-99049-img0003.jpg "Immunohistochemistry: IKK alpha Antibody [NBP2-99049] - Immunochemical staining of human IKK in human colon carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: IKK alpha Antibody [NBP2-99049] - Immunochemical staining of human IKK alpha in human breast carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-99049-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: IKK alpha Antibody [NBP2-99049] - Immunofluorescence staining of IKK alpha in HCT116 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99049 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-99049_rabbit-polyclonal-ikk-alpha-antibody-2352025166478.png)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (511) [NBP2-89187] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-511-Immunocytochemistry-Immunofluorescence-NBP2-89187-img0001.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (511) [NBP2-89187] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.")
![Flow Cytometry: ErbB2/Her2 Antibody (511) [NBP2-89187] - Analysis of anti-ErbB2 on MCF-7 cells. MCF-7 cells were detached using 1X trypsin, washed, then stained with purified rabbit anti-human ErbB2. Second step staining with FITC conjugated goat anti-rabbit IgG (H+L) polyclonal antibody.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-511-Flow-Cytometry-NBP2-89187-img0003.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (511) [NBP2-89187] - Analysis of anti-ErbB2 on SK-BR3 cells. SK-BR3 cells were detached using 1X trypsin, washed, then stained with Purified Rabbit anti-Human ErbB2. Second step staining with FITC conjugated Goat anti-Rabbit IgG polyclonal antibody (BD Pharmingen (R).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-511-Flow-Cytometry-NBP2-89187-img0002.jpg)
![Western Blot: CRIPTO Antibody (JE33-31) [NBP3-32221] - Western blot analysis of CRIPTO on NCCIT cell lysates with Rabbit anti-CRIPTO antibody (NBP3-32221) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32221) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32221_rabbit-cripto-mab-je33-31-278202416285240.jpg "Western Blot: CRIPTO Antibody (JE33-31) [NBP3-32221] - Western blot analysis of CRIPTO on NCCIT cell lysates with Rabbit anti-CRIPTO antibody (NBP3-32221) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32221) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: CRIPTO Antibody (JE33-31) [NBP3-32221] - Immunocytochemistry analysis of NCCIT cells labeling CRIPTO with Rabbit anti-CRIPTO antibody (NBP3-32221) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CRIPTO antibody (NBP3-32221) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32221_rabbit-cripto-mab-je33-31-2920241114828.jpg)
![Flow Cytometry: CRIPTO Antibody (JE33-31) [NBP3-32221] - Flow cytometric analysis of NCCIT cells labeling CRIPTO. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32221, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32221_rabbit-cripto-mab-je33-31-392024884631.jpg)
![Western Blot: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Western blot analysis of ErbB2/Her2 on different lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 10 minutes cell lysate Lane 3: A431 treated with 100ng/mL EGF for 10 minutes cell lysate, then the membrane treated with pp for 1 hour Lane 4: SK-Br-3 cell lysate Lane 5: SK-Br-3 starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 138 kDa Observed band size: 180 kDa Exposure time: 10 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32768) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32768_rabbit-erbb2-her2-p-tyr1139-mab-je60-90-288202412442922.jpg "Western Blot: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Western blot analysis of ErbB2/Her2 on different lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 10 minutes cell lysate Lane 3: A431 treated with 100ng/mL EGF for 10 minutes cell lysate, then the membrane treated with pp for 1 hour Lane 4: SK-Br-3 cell lysate Lane 5: SK-Br-3 starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 138 kDa Observed band size: 180 kDa Exposure time: 10 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32768) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Immunocytochemistry analysis of A431 cells treated with or without 100ng/mL EGF for 10 minutes labeling ErbB2/Her2 with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/5,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/5,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32768_rabbit-erbb2-her2-p-tyr1139-mab-je60-90-29202410484847.jpg)
![Flow Cytometry: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Flow cytometric analysis of A431 cells treated with or without 100ng/mL EGF for 10 minutes labeling ErbB2/Her2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32768, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32768_rabbit-erbb2-her2-p-tyr1139-mab-je60-90-2920241637217.jpg)
![Western Blot: PKC gamma Antibody (A12-H) [NBP1-30124] - Left Lane: Marker, Right Lane: Mouse brain crude lysate (200ug of protein loaded).](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-A12-H-Western-Blot-NBP1-30124-img0001.jpg "Western Blot: PKC gamma Antibody (A12-H) [NBP1-30124] - Left Lane: Marker, Right Lane: Mouse brain crude lysate (200ug of protein loaded).")
![Immunocytochemistry/Immunofluorescence: PKC gamma Antibody (A12-H) [NBP1-30124] - PKC-gamma expression in HEK293 cells.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-A12-H-Immunocytochemistry-Immunofluorescence-NBP1-30124-img0003.jpg)